Gitanjali Rao is born in Denver, Colorado. Details about her early childhood are limited, but it's evident that she had access to resources and encouragement that fostered her curiosity.

During her early elementary years, Rao displayed a keen interest in science and technology. She actively participated in STEM-related activities, suggesting a natural inclination towards problem-solving and innovation.

Rao reportedly began experimenting with various scientific concepts and technologies around the age of 10. This period was crucial for developing her practical skills and fostering a hands-on approach to learning.

Rao received formal STEM education in middle school. The specifics of her curriculum are not entirely known, but it likely included courses in mathematics, science, and technology.

Inspired by the Flint water crisis and a desire to solve the problem of lead contamination, Rao began working on a solution to detect lead in water faster and more effectively than existing methods. This was a pivotal moment in her development as an inventor.

Continued work on Tethys, her device for detecting lead in water. This involved extensive research, experimentation, and iterations. She began to seek out mentorship and opportunities to showcase her invention.