The globulins are a family of globular proteins that have higher molecular weights than albumins and are insoluble in pure water but dissolve in dilute salt solutions. Some globulins are produced in the liver, while others are made by the immune system. Globulins, albumins, and fibrinogen are the major blood proteins. The normal concentration of globulins in human blood is about 2.6-3.5 g/dL.

The term "globulin" is sometimes used synonymously with "globular protein". However, albumins are also globular proteins, but are not globulins. All other serum globular proteins are globulins.

All globulins fall into one of three categories:

Globulins can be distinguished from one another using serum protein electrophoresis.

Globulins exert oncotic pressure. Their deficiency results in loss of carrier functions of globulins, oedema due to decreased oncotic pressure, and susceptibility to infections due to decreased gamma-globulins (immuno-globulins) leading to decreased production of antibodies.

Globulins are mainly divided into three different sections depending on their electrophoretic mobility. The main sections for globulin would be the alpha globulins, beta globulin, and gamma globulin. The alpha globulins and the beta globulin are mainly created in the liver and the gamma globulin are made by lymphocytes and plasma cells in lymphoid tissue.

These globulins should consist of non-albumin proteins and there could be about a hundred different proteins that are included in the globulins. One group of proteins that are in the globulins is created in return for the inflammatory stimuli.

There is a certain equation to figure out the value of globulin. The equation goes by this Globulins = Total protein - Albumin since it should not have an albumin protein within the globulin. Usually, the concentration of globulin is measured either in grams per liter or milligrams per deciliter.