Gramicidin, also called gramicidin D, is a mix of ionophoric antibiotics, gramicidin A, B and C, which make up about 80%, 5%, and 15% of the mix, respectively. Each has 2 isoforms, so the mix has 6 different types of gramicidin molecules. They can be extracted from Brevibacillus brevis soil bacteria. Gramicidins are linear peptides with 15 amino acids. This is in contrast to unrelated gramicidin S, which is a cyclic peptide.

Gramicidins work as antibiotics against gram-positive bacteria like Bacillus subtilis and Staphylococcus aureus, but not well against gram-negative ones like E. coli.

Gramicidins are used in medicinal lozenges for sore throat and in topical medicines to treat infected wounds. Gramicidins are often mixed with other antibiotics like tyrocidine and antiseptics. Gramicidins are also used in eye drops for bacterial eye infections. In drops, they are often mixed with other antibiotics like polymyxin B or neomycin. Multiple antibiotics increase efficiency against various strains of bacteria. Such eye-drops are also used to treat eye infections of animals, like horses.

In 1939, René Dubos isolated the substance tyrothricin. Later this was shown to be a mix of gramicidin and tyrocidine. These were the first antibiotics to be manufactured commercially. Letter "D" in gramicidin D is short for "Dubos", and was invented to differentiate the mix from gramicidin S.

In 1964, the sequence of gramicidin A was determined by Reinhard Sarges and Bernhad Witkop.

In 1971, the dimeric head-to-head structure of gramicidins was proposed by D. W. Urry.

In 1993, the structure of the gramicidin head-to-head dimer in micelles and lipid bilayers was determined by solution and solid-state NMR.

Gramicidins A, B and C are nonribosomal peptides, thus they have no genes. They consist of 15 L- and D-amino acids. Their amino acid sequence is: