Harbour Grace is a town in Conception Bay on the Avalon Peninsula in the province of Newfoundland and Labrador, Canada. With roots dating back to the 16th century, it is one of the oldest towns in North America.

It is located about 90 kilometres (56 mi) northwest of the provincial capital, St. John's. The town has a population of 2,796 (2021), engaged primarily in fishing and fish processing. The alternative spelling of Harbor Grace was current at one time.

The town was named after the French city of Havre de Grâce, which is now known as Le Havre. Harbour Grace was an important port and fishing centre from the earliest days of European exploration of North America. The town was a thriving seasonal fishing community by 1550. The first resident known by name was Robert Tossey of Dartmouth in 1583.

In 1610, pirate Peter Easton made Harbour Grace his headquarters, and established a fort overlooking the bay. Although it was attacked by the French the following year, the early settlement survived throughout the 17th century, with a permanent, year-round population numbering a few dozen, swelling to several hundred during the fishing season.

Around the year 1618, Harbour Grace became a permanent settlement. In that year 1618, Bristol's Society of Merchant Venturers received a charter from King James I of England to establish a settlement near Harbour Grace, "Bristol's Hope", and appointed Robert Hayman as its first Proprietary Governor, a post he held for the next ten years. He was back in London at the end of this period in 1628, where he published a work of pithy epigrams called Quodlibets. He had written this in Harbour Grace;

Over the coming years, control of Harbour Grace became a point of contention between the English and the French. The town, with a population numbering about 100, was razed by the French in 1697, again in 1700, and captured briefly in 1762. Nevertheless, between these attacks, the population grew by 50%. By 1771, the population was close to 5,800. By then, however, other colonial towns along the Atlantic coast had surpassed Harbour Grace in population and influence.

The Gordon G. Pike Railway Heritage Museum and Park (c. 1881–1884) was designated a Municipal Heritage Building in 2006. The first railway line in Newfoundland was completed to Harbour Grace in 1884.

The Harbour Grace Court House, constructed in 1830, is the oldest surviving public building in the province and a National Historic Site of Canada. St. Paul's Anglican Church in Harbour Grace was built in 1835, making it the oldest stone church in Newfoundland and Labrador. Built around 1867, the Masonic building of Lodge Harbour Grace No. 476 A.F. & A.M. S.C., is the oldest wooden Masonic meeting house in Canada. The 1870 customs house is now the Conception Bay Museum