Hartismere Rural District was a rural district in the county of East Suffolk, England. It was expanded in 1934 by merging with the disbanded Hoxne Rural District with a slight readjustment of boundaries. It was named after the ancient Hundred of Hartismere and administered from Eye.

Since 1 April 1974 it has formed part of the District of Mid Suffolk.

At the time of its dissolution it consisted of 52 civil parishes.

52°17′N 1°16′E / 52.28°N 1.27°E / 52.28; 1.27