John Kuriyan is an American structural biologist and biochemist. He is the dean of basic sciences and a professor of biochemistry at Vanderbilt University School of Medicine. He was previously a professor at the University of California, Berkeley and an investigator of the Howard Hughes Medical Institute. He was elected to the National Academy of Sciences in 2001 and to the National Academy of Medicine in 2018.

Kuriyan received his B.S. in chemistry from Juniata College in Pennsylvania, followed by his Ph.D. in physical chemistry at the Massachusetts Institute of Technology supervised by Gregory Petsko and Martin Karplus.

Kuriyan did postdoctoral research for one year with Martin Karplus at Harvard University before becoming an assistant professor at Rockefeller University. Kuriyan's laboratory studies the structure and mechanism of enzymes and other proteins involved in cellular signal transduction and DNA replication.

Kuriyan's research has focused on the structural basis of protein kinase regulation and signaling, including mechanisms of activation and inhibition in tyrosine kinases. This is based in part on the use X-ray crystallography. His work has also contributed to methodological advances in macromolecular structure determination, including the use of molecular dynamics in crystallographic refinement.

In 1989, Kuriyan was named a Pew Scholar. He received the Schering-Plough Award of the American Society for Biochemistry and Molecular Biology (1994), the Dupont–Merck Award of the Protein Society (1997), the Eli Lilly Award in Biological Chemistry of the American Chemical Society (1998), and the Cornelius Rhoads Memorial Award from the American Association for Cancer Research (1999). He was awarded the Richard Lounsbery Award of the National Academy of Sciences in 2005. In 2009 he received the ASBMB Merck Award for his contributions to structural biology. Kuriyan was elected a Foreign Member of the Royal Society in 2015. He was elected to the National Academy of Medicine in 2018.