Sir John de Pulteney (sometimes spelled Poultney; died 8 June 1349) was a major English entrepreneur and property owner, who served four times as Lord Mayor of London.

A biography of Sir John, written by Charles Lethbridge Kingsford, that was published in the Dictionary of National Biography, 1885–1900, Volume 47 contains much well-referenced information. However, it is inaccurate in stating the Sir John's wife was a daughter of John de St John of Lageham, for reasons that are set out in detail in two articles by Walter Lee Sheppard Jr.

Sir John's parents are identified in his will as Adam and Matilda.

Sir John de Pulteney married Margaret, daughter of John de Bereford, citizen of London and his wife Roesia. The marriage evidently took place by 13 December 1330, when they arranged for perpetual masses for themselves and “for the souls of John de Bureford late citizen of London and Roesia his wife” to take place at St Nicholas Shambles, London, since demolished. His association with the de Bereford family went back significantly before 1330, as in July 1318 he was recorded as executor of the will of Roesia de Bureford, late the executrix of John de Bureford, citizen of London.

The couple had one known child, William de Pulteney (1340-1366/67).

Shortly after Sir John de Pulteney's death, his widow married Sir Nicholas de Loveyne.

He was buried in Old St Paul's Cathedral, but the grave and monument were destroyed along with the cathedral in the Great Fire of London in 1666. A modern monument in the crypt lists his among the important graves lost, shown above right.

Sir John was a citizen of London and a member of the Drapers’ Company.