The Johnson City Cardinals were a Minor League Baseball team based in Johnson City, Tennessee. The team was affiliated with the St. Louis Cardinals organization from 1975 through 2020 and played in the Rookie-level Appalachian League. The team won 10 league championships, most recently in 2019. They played their home games at TVA Credit Union Ballpark.

The start of the 2020 season was postponed due to the COVID-19 pandemic before ultimately being cancelled on June 30. In conjunction with a contraction of Minor League Baseball beginning with the 2021 season, the Appalachian League was reorganized as a collegiate summer baseball league, and the Cardinals were replaced by the Johnson City Doughboys, a new franchise in the revamped league designed for rising college freshmen and sophomores.

From 1986 to 2019, Johnson City reached the playoffs by virtue of winning their division or clinching a playoff spot eight times. The results of their postseason play during this time was as follows.

Many of the players fielded by Johnson City have gone on to distinguish themselves in Major League Baseball.