Killarney Rugby Football Club is a rugby union club based in Killarney, County Kerry, Ireland. It is affiliated to the Munster Branch of the Irish Rugby Football Union. The club competes in Division 3 of the Munster Junior League. While a club existed in the town as far back as the end of the 19th century it wasn't until 1983 that the club in its current format was founded. Killarney currently have one senior sides as well as a large underage and mini set-up, and runs a successful Tag Rugby league during the summer months.

Killarney's earliest recorded links with rugby date back to the 1800s, notable players of the era were Jack McKay and MacGillycuddy of the Reeks. Early matches were played in the Cricket Field, a piece of ground on the northern banks of the Flesk opposite the Gleneagle pitch and putt course.

The earliest recorded game is detailed in the archives of Laune Rangers GAA, which recounts a match between Killarney and Killorglin in January 1888 "the beginning" followed by a return in Killorglin the following month.

This was not a one-off as there are newspaper reports the following year of a match between Tralee and Killarney on Thursday, 17 January 1889. The score was one try a piece.

Whilst there are no records of an official club being founded before this time, there are reports of a "large and enthusiastic public meeting" being held on 22 November 1898 to revive Killarney Rugby Football Club. According to records from the time this meeting got a great response and within a couple of months rugby was once again flourishing in Killarney.

In 1928 that Killarney RFC was again reformed after an interested group managed to secure a playing field at Countess Road from the Earl of Kenmare. The club was founded at a meeting in the Commercial Club, New St., Killarney on 28 April 1928. At the time the club had players who had senior experience with clubs such as Cork Constitution, Dolphin, Sunday's Well and U.C.C. They enjoyed relative success for a number of years but by the mid-1930s things began to decline. Initially emigration and then the Emergency resulted in the club ceasing to function. However a number of locals did form a team in 1941 and won the Galwey-Foley Cup beating Castleisland in the Final. It was almost 20 years before the club was re-established again.

The club was established again in time for the 1953/54 season and almost immediately enjoyed success which continued right up to the 1970s. The club won both the McGillycuddy Cup and Galwey-Foley Cup in both 1954/55 and 1955/56 and also reached the semi-finals of the Munster Junior Cup in 1955/56. Also that season Welsh side Aberavon visited and played Killarney. The following year they lost the Cork County Cup Final to Cobh Pirates but became the first Kerry side to tour 'overseas' when they travelled to Wales to play Port Talbot and Margan.

Killarney won the Aberavon Shield in 1957/58 and the following season they were involved in an epic Galwey-Foley Cup Final encounter with their neighbours and great rivals Castleisland. The sides could not be separated after three matches including extra-time. The club won the Hayes Cup in 1959/60 and again in 1961/62. The club continued to have success until it was wound up in the early 1970s.