Lothian and Borders Police was the territorial police force for the Scottish council areas of the City of Edinburgh, East Lothian, Midlothian, Scottish Borders and West Lothian between 1975 and 2013. The force's headquarters were in Fettes Avenue, Edinburgh.

Lothian and Borders Police was formed on 16 May 1975 by an amalgamation of Berwick, Roxburgh and Selkirk Constabulary, Edinburgh City Police and The Lothians and Peebles Constabulary.

The force had 2,905 officers and 1,384 support staff as of March 2008. The force's last Chief Constable was David Strang who replaced Paddy Tomkins on 29 March 2007.

An Act of the Scottish Parliament, the Police and Fire Reform (Scotland) Act 2012, created a single Police Service of Scotland—known as Police Scotland—with effect from 1 April 2013. This merged the eight former regional police forces in Scotland (including Lothian & Borders Police), together with the Scottish Crime and Drug Enforcement Agency, into a single service covering the whole of Scotland. Police Scotland has its headquarters at the Scottish Police College at Tulliallan in Fife.

The Lothian and Borders Police area stretched from Blackridge in the west to Newcastleton in the south. It was split into four territorial divisions, and several other non-territorial divisions for specialist and administrative roles.

A Division covered the City of Edinburgh council area, and was created in 2002 following the amalgamation of the City of Edinburgh's three previous divisions. It was the largest territorial division in terms of manpower and population. Its headquarters were St Leonards Police Station.

E Division covered East Lothian and Midlothian, stretching from the Edinburgh City Bypass to Dunbar in the east. Its headquarters were in Dalkeith.

F Division covered West Lothian with its headquarters in Livingston.