A lunar regolith simulant is a terrestrial material synthesized in order to approximate the chemical, mechanical, engineering, mineralogical, or particle-size distribution properties of lunar regolith. Lunar regolith simulants are used by researchers who wish to research the materials handling, excavation, transportation, and uses of lunar regolith. Samples of actual lunar regolith are too scarce, and too small, for such research, and have been contaminated by exposure to Earth's atmosphere.

In the run-up to the Apollo program, crushed terrestrial rocks were first used to simulate the anticipated soils that astronauts would encounter on the lunar surface. In some cases the properties of these early simulants were substantially different from actual lunar soil, and the issues associated with the pervasive, fine-grained, sharp dust grains on the Moon came as a surprise.

After Apollo and particularly during the development of the Constellation program, there was a large proliferation of lunar simulants produced by different organizations and researchers. Many of these were given three-letter acronyms to distinguish them (e.g., MLS-1, JSC-1), and numbers to designate subsequent versions. These simulants were broadly divided into highlands or mare soils, and were usually produced by crushing and sieving analogous terrestrial rocks (anorthosite for highlands, basalt for mare). Returned Apollo and Luna samples were used as reference materials in order to target specific properties such as elemental chemistry or particle size distribution. Many of these simulants were criticized by prominent lunar scientist Larry Taylor for a lack of quality control and wasted money on features like nanophase iron that had no documented purpose.

JSC-1 (Johnson Space Center Number One) was a lunar regolith simulant that was developed in 1994 by NASA and the Johnson Space Center. Its developers intended it to approximate the lunar soil of the maria. It was sourced from a basaltic ash with a high glass content.

In 2005, NASA contracted with Orbital Technologies Corporation (ORBITEC) for a second batch of simulant in three grades:

NASA received 14 metric tons of JSC-1A, and one ton each of AF and AC in 2006. Another 15 tons of JSC-1A and 100 kg of JSC-1F were produced by ORBITEC for commercial sale, but ORBITEC is no longer selling simulants and was acquired by the Sierra Nevada Corporation. An 8-ton sand box of commercial JSC-1A is available for daily rental from the NASA Solar System Exploration Research Virtual Institute (SSERVI).

JSC-1A can geopolymerize in an alkaline solutions resulting in a hard, rock-like, material. Tests show that the maximum compressive and flexural strength of the 'lunar' geopolymer is comparable to that of conventional cements.

JSC-1 and JSC-1A are now no longer available outside of NASA centers. ^{[citation needed]}