Madonna University is a private Catholic university in Livonia, Michigan, United States. It was founded as the Presentation of the Blessed Virgin Mary Junior College by the Felician Sisters in 1937. It became Madonna College in 1947 and Madonna University in 1991.

Among the largest Franciscan universities in the country, Madonna is situated on an 80-acre (32 ha) wooded campus. It has extension campuses in southwest Detroit, Orchard Lake, and Clinton Township at the Macomb University Center. Madonna University also has a campus at the University Center in Gaylord, about 225 miles (362 km) north of Livonia.

Founded by two sisters in 1937 as Presentation of the Blessed Virgin Mary College, the university offers more than 100 undergraduate majors toward associate and bachelor's degrees as well as 35 master's programs in clinical psychology, business, criminal justice, education, history, and health professions. In May 2009, Madonna began offering its first doctorate, the Doctor of Nursing Practice. Madonna's most popular undergraduate majors, by 2021 graduates, were:

Madonna University's commitment to developing a sustainable and eco-friendly campus was recognized when the U.S. Green Building Council awarded Gold level LEED® (Leadership in Energy and Environmental Design) certification for the university's 60,000 square feet (5,600 m²) Franciscan Center.

The university is organized into the following colleges and schools:

The Madonna Now is the university's magazine for alumni and friends. The Madonna Herald is the university's newspaper, which is produced by Madonna's Journalism Department, headed by professor Neal Haldane. The Madonna Muse is an annual literary journal.

In the university's Franciscan Center studios, students operate an online radio station at Live 365. The format is freeform, and features occasional live broadcasts from students of the program.

The Broadcast and Cinema Arts students produce a television show, Celebrate Michigan, which airs on MyTV20 in the Detroit area.