The Legislative Assembly of Manitoba (French: Assemblée législative du Manitoba) is the deliberative assembly of the Manitoba Legislature in the Canadian province of Manitoba. Fifty-seven members are elected to this assembly at provincial general elections, all in single-member constituencies with first-past-the-post voting. Bills passed by the Legislative Assembly are given royal assent by the lieutenant governor of Manitoba in the name of the King of Canada. The Manitoba Legislative Building is located in central Winnipeg.

The premier of Manitoba is Wab Kinew, and the speaker of the Legislative Assembly of Manitoba is Tom Lindsey. Both are members of the New Democratic Party.

Historically, the Legislature of Manitoba had another chamber, the Legislative Council of Manitoba, but this was abolished in 1876, just six years after the province was formed.

The 42nd Legislature was dissolved on September 5, 2023, and a general election was called for October 3. The 43rd Legislature opened on November 9.

The seating arrangement is viewable at the official website.