Mariners' Church of Detroit is a church with worship services adhering to Anglican liturgical traditions located at 170 East Jefferson Avenue in Downtown Detroit. It was founded in 1842 as a special mission to the maritime travelers of the Great Lakes and functioned as a parish of the Episcopal Diocese of Michigan until 1992, when the Michigan Court of Appeals ruled it was incorporated as an independent congregation. The church was listed on the National Register of Historic Places in 1971. The church has a non-exclusive, limited association with the REC Diocese of Mid-America which itself is a founding member of the Anglican Church in North America.

The church grounds contain a bronze sculpture of George Washington wearing the apron of a master mason by Donald De Lue. The sculpture is a copy of the wax original created in 1959 and was installed in 1966. The statue belongs to the City of Detroit, not the church; the site was chosen because it is the location of the first Council House in Detroit.

The church was founded as a result of Julia Anderson's bequest of the lot on which her own mansion stood, at the corner of Woodbridge and Woodward streets. The completion of the Erie Canal increased shipping traffic in the area, and Anderson felt that the seamen were in need of spiritual support and care. Mariners' was established in 1842 and incorporated in the state of Michigan in 1848; the present structure was consecrated the following year.

The original wood-frame church was sold to the congregation of Historic Trinity Lutheran Church for $200 when the current structure was completed. The Trinity congregation moved the building to Larned and Rivard streets.

Prior to the Civil War, the church was a stop on the Underground Railroad which black slaves followed North to Canada and freedom. In 1925, the church established Mariners' Inn, a shelter that now offers rehabilitation, counseling and vocational services for homeless substance abusers.

The church was slated for demolition along with many neighboring structures in 1955 to make room for the new civic center. Trustee and The Detroit News columnist George Stark urged his readers to help save the historic building and asked for small contributions of $5 or $10 to move the 60,000 pounds (27,000 kg) limestone edifice 880 feet (270 m) east. The undertaking was detailed in the April 25, 1955, issue of Life magazine. Stark's appeal raised enough money to cover moving costs with money left to add stained glass windows, a west entry and a tower and to redecorate the interior of the church. During the move, workers discovered a tunnel under the building from its days as a stop on the Underground Railroad. It now sits atop part of the Detroit–Windsor Tunnel.

The new site for the church on Jefferson Avenue was where the Indian Council House was constructed after the 1805 fire. The house was used for meetings with local tribes and the first Protestant worship services in Detroit. It later was the site of the Army Corps of Engineers Topographical Corps offices. The office was established by Col. John Anderson, husband of Julia Anderson, Mariners' benefactress.

In August 1990, the Episcopal Diocese of Michigan filed a lawsuit claiming jurisdiction over Mariners' Church as a parish within the Episcopal Church. Mariners' Church held that it is an independent congregation free of denominational ties, while the Episcopal Church held that it had always considered the church an Episcopal congregation. The courts ruled in favor of Mariners' Church in the initial case in 1990 and an appeal in 1992.