Melrose Rugby Football Club is a rugby union club located in the town of Melrose in the Scottish Borders. The men's side competes in the Scottish Premiership. The club plays at the Greenyards. The club is synonymous with inventing Rugby sevens and their tournament the Melrose Sevens is one of the most prestigious sevens tournaments in the world.

Between the 2019-2020 and 2023-2024 seasons the club ran a men's professional side known as the Southern Knights which competed in the Super 6 league and Super Sprint competitions.

The club was formed in 1877 and was elected to full membership of the Scottish Rugby Union in 1880. The club have played at the Greenyards since the club's inception.

Melrose have been Scottish champions on ten occasions and Scottish cup winners four times. They lifted the Scottish Cup in 1997 to complete a domestic double and have also won the Border League on 17 occasions.

Despite the population of Melrose hovering around 2000 for a number of years the team has been a consistently successful club in the upper echelons of the Scottish game.

Edinburgh Rugby players drafted:

As well as the Super 6 and 1st XV, Melrose have a youth side (Melrose WASPS) and a reserve side (Melrose Storm). The club had a successful Ladies side until 2018.

Melrose Ladies was one of the top women's teams in Scotland, competing in the Scottish Premiership and the Scottish Cup. The team produced a number of players who went on to represent Scotland. Four members of the current Scotland squad – Lisa Thomson, Lana Skeldon, Lauren Harris and Chloe Rollie – played at Melrose. The team folded in 2018.