The unicameral Assemblée nationale or National Assembly is Guinea's legislative body. Since the country's birth in 1958, it has experienced political turmoil, and elections have been called at irregular intervals, and only since 1995 have they been more than approval of a one-party state's slate of candidates. The number of seats has also fluctuated.

It is currently suspended, with the National Council of the Transition acting as Guinea's legislative body in the wake of the 2021 Guinean coup d'état.

The new constitution of 2025 outlines a bicameral legislature with a lower chamber, National Assembly, of 147 seats who are directly elected for five years. The upper chamber, Senate, is indirectly elected for a term of six years.

The National Assembly will have 147 seats, 49 of them are directly elected based on a national list on proportional representation. The remaining 98 seats are elected on constituency level.

The first legislative arm was Guinean Territorial Assembly in French Guinea in 1952. After independence, a new unicameral National Assembly was established in 1958. Saifoulaye Diallo was the first President of the National Assembly. Single-party rule was quickly established by Ahmed Sékou Touré. The National Assembly was reformed as Popular Revolutionary Assembly in 1974. The legislature was dissolved in the 1984 coup by colonel Lansana Conté, who ruled by decree.

A new unicameral National Assembly with 114 seats was established in 1995, along with first multiparty elections in Guinea. The National Assembly was disbanded after the 2008 coup. A temporary National Transitional Council worked from 2010 to 2014. A new unicameral National Assembly with 114 seats worked from 2014 until the 2021 coup. A temporary National Council of Transition was established in 2022 under the junta with 81 seats. A new constitution approved in the 2025 constitutional referendum outlines a bicameral parliament with a popularly elected National Assembly and an indirectly elected less powerful Senate.

Two thirds of the members (76), called députés, are directly elected through a system of proportional representation, using national party-lists, while one third (38) are elected from single-member constituencies, using the simple majority (or first-past-the-post) system. Members must be over 25 years old and serve five-year terms.

The President of the National Assembly of Guinea is the presiding officer of the legislature. Claude Kory Kondiano has been President of the National Assembly since January 2014.