A neutral sixth is a musical interval wider than a minor sixth play^ⓘ but narrower than a major sixth play^ⓘ. Three distinct intervals may be termed neutral sixths:

These intervals are all within about 12 cents of each other and are difficult for most people to distinguish. Neutral sixths are roughly a quarter tone sharp from 12 equal temperament (12-ET) minor sixths and a quarter tone flat from 12-ET major sixths. In just intonation, as well as in tunings such as 31-ET, 41-ET, or 72-ET, which more closely approximate just intonation, the intervals are closer together.

A neutral sixth can be formed by subtracting a neutral second from a minor seventh. Based on its positioning in the harmonic series, the undecimal neutral sixth implies a root one minor seventh above the higher of the two notes.

The pitch ratio 13:8 (840.53 cents) is the ratio of the thirteenth harmonic and is notated in Ben Johnston's system as A¹³♭. In 24-ET is approximated by A. This note is often corrected to a just or Pythagorean ratio on the natural horn, but the pure thirteenth harmonic was used in pieces including Britten's Serenade for tenor, horn and strings.