Trunk 28 is part of the Canadian province of Nova Scotia's system of trunk highways. The route runs from Sydney to Glace Bay, a distance of 36 kilometres (22 mi).

From near downtown Sydney, Trunk 28 takes Victoria Road northward through the Whitney Pier neighbourhood, continuing along Sydney Harbour through South Bar, New Victoria and Victoria Mines to New Waterford. Trunk 28 turns south towards Lingan Bay, then east through Dominion entering Glace Bay in the Bridgeport area and following Main Street and terminating downtown at the intersection with Commercial Street.