In physics and astronomy, a frame of reference (or reference frame) is an abstract coordinate system, whose origin, orientation, and scale have been specified in physical space. It is based on a set of reference points, defined as geometric points whose position is identified both mathematically (with numerical coordinate values) and physically (signaled by conventional markers). An important special case is that of an inertial reference frame, a stationary or uniformly moving frame.

For n dimensions, n + 1 reference points are sufficient to fully define a reference frame. Using rectangular Cartesian coordinates, a reference frame may be defined with a reference point at the origin and a reference point at one unit distance along each of the n coordinate axes.^{[citation needed]}

In Einsteinian relativity, reference frames are used to specify the relationship between a moving observer and the phenomenon under observation. In this context, the term often becomes observational frame of reference (or observational reference frame), which implies that the observer is at rest in the frame, although not necessarily located at its origin. A relativistic reference frame includes (or implies) the coordinate time, which does not equate across different reference frames moving relatively to each other. The situation thus differs from Galilean relativity, in which all possible coordinate times are essentially equivalent.^{[citation needed]}

The need to distinguish between the various meanings of "frame of reference" has led to a variety of terms. For example, sometimes the type of coordinate system is attached as a modifier, as in Cartesian frame of reference. Sometimes the state of motion is emphasized, as in rotating frame of reference. Sometimes the way it transforms to frames considered as related is emphasized as in Galilean frame of reference. Sometimes frames are distinguished by the scale of their observations, as in macroscopic and microscopic frames of reference.

In this article, the term observational frame of reference is used when emphasis is upon the state of motion rather than upon the coordinate choice or the character of the observations or observational apparatus. In this sense, an observational frame of reference allows study of the effect of motion upon an entire family of coordinate systems that could be attached to this frame. On the other hand, a coordinate system may be employed for many purposes where the state of motion is not the primary concern. For example, a coordinate system may be adopted to take advantage of the symmetry of a system. In a still broader perspective, the formulation of many problems in physics employs generalized coordinates, normal modes or eigenvectors, which are only indirectly related to space and time. It seems useful to divorce the various aspects of a reference frame for the discussion below. We therefore take observational frames of reference, coordinate systems, and observational equipment as independent concepts, separated as below:

Although the term "coordinate system" is often used (particularly by physicists) in a nontechnical sense, the term "coordinate system" does have a precise meaning in mathematics, and sometimes that is what the physicist means as well.

A coordinate system in mathematics is a facet of geometry or of algebra, in particular, a property of manifolds (for example, in physics, configuration spaces or phase spaces). The coordinates of a point r in an n-dimensional space are simply an ordered set of n numbers:

In a general Banach space, these numbers could be (for example) coefficients in a functional expansion like a Fourier series. In a physical problem, they could be spacetime coordinates or normal mode amplitudes. In a robot design, they could be angles of relative rotations, linear displacements, or deformations of joints. Here we will suppose these coordinates can be related to a Cartesian coordinate system by a set of functions: