The Padang Besar railway station (Malay: Stesen keretapi Padang Besar) is a railway station located at and named after the town of Padang Besar, Perlis in Malaysia. The town is located at the Malaysia-Thailand border.

It is the terminus of the KTM West Coast railway line, and connects to the State Railway of Thailand's rail network via its Southern Line at this station.

This station is located about 200 metres south of the actual border between Malaysia and Thailand. This station should not be confused with another station, named Padang Besar (Thai), which is located in the Thai town of Padang Besar and fully operated by SRT. The two stations are about 400 metres apart.

Despite being in Malaysian territory, owned by Malaysia's Railway Asset Corporation and operated by Keretapi Tanah Melayu (KTM), the station is served by both Malaysian and Thai railway services, allowing for passengers to transfer between the two railway systems. SRT operates a ticket office for their trains operating from the station and accept both Malaysian Ringgit and Thai Baht for ticket purchases. Malaysian and Thai immigration, customs and quarantine checkpoints are also co-located within this station specifically for cross-border train passengers.

Padang Besar railway station also has a freight yard that serves as a dry port for the northern part of Malaysia and Indonesia–Malaysia–Thailand Growth Triangle.

The station can be accessed by road which is linked to Federal Route 7 just outside Padang Besar town. The station can also be accessed via a pedestrian walkway which links the station to Padang Besar town as well as the Malaysian immigration, customs, quarantine and security checkpoint for the road border crossing. Taxis and cross-border van services to the Thai city of Hat Yai are available from the station.

Padang Besar railway station is served by trains operated by both Keretapi Tanah Melayu (KTM) and the State Railway of Thailand (SRT).

Train services by Keretapi Tanah Melayu serving Malaysian destinations are: