The Pittsburgh Colts are the oldest minor-league professional football team, located in Pittsburgh, Pennsylvania, that is still in existence. Since 2018 they have been playing an independent schedule.

The team was founded in 1979 by Ed Brosky, who either played for or coached the Colts in every season since. Since its inception, 32 players who played for the Colts have received a professional contract.

The team was founded in 1979 by head coach Edward G. Brosky, a member of the Pittsburgh Panthers 1976 national championship team, and played in the Keystone Oaks area. Brosky also played as a semi-pro football player while playing college football, until then-Panthers coach Johnny Majors found out and ended Brosky's moonlighting. When he left Pitt, Brosky continued to play for semi-pro teams in the area. He started the Colts when those other teams folded.

The Colts initial run lasted from 1979 until 1987, when the team took a four-year hiatus. In that span, the team reached the Minor Professional Football Association minor league championship in 1981 to play against the Twin City Cougars, who later became the Oakland Invaders of the United States Football League. The team also played one season as a pro team, when they were member of the American Football Association in that league's final season.

In the mid-1990s, Brosky struck a deal with the Northgate School District. It was during this time that two notables were linked with the team; WTAE-TV field reporter Sheldon Ingram played in the defensive backfield while future music video director Matt Dilmore served as the team's official announcer. The team draw talent from the area high schools and local colleges including: Allegheny College, Bethany College, California University of Pennsylvania, Carnegie Mellon University, Duquesne University, Indiana University of Pennsylvania, University of Pittsburgh, Robert Morris College, Slippery Rock University, Washington & Jefferson College, and James Madison University in Virginia. The Colts describe themselves as a "throwback team" based on a strong work ethic and personal values. The Colts have interacted with the community they are in through numerous youth activities, visiting children's hospital, and having community events downtown.

The team briefly disbanded again after the 2000 season due its board of directors and coaching staff have become the ownership group of the Pittsburgh Ironmen, a new professional indoor football team set to begin competing in February 2001.

The Colts were previously members of the American, North American Football League, and Regional American Football League before Edward Brosky formed the Grassroots Football League in 2009. The Colts played in the Semi-Pro Football League from 2010 to 2014 before joining the Gridiron Developmental Football League for the 2015 season. They also played at Chartiers Valley for the 2015 season.

In 2004 Lisa Horton became the first woman to throw a touchdown pass in a men's America Football League game.