Resident Evil Outbreak: File #2 is a survival horror video game developed and published by Capcom with online playability for the PlayStation 2. It is the sequel to Resident Evil Outbreak, and the final installment of the spin-off series. It was released on September 9, 2004 in Japan, on April 26, 2005 in North America, and August 26, 2005 in PAL regions.

After the success of Outbreak in Japan, Capcom announced a sequel in Fall 2004. The game takes place in zombie-infested Raccoon City with the same eight characters that were featured in its predecessor, along with five new scenarios.

Upon release, File #2 featured online multiplayer servers, but in March 2007 all servers shut down. On January 1, 2014, alternate fan servers restored online play, along with new additions to the servers, such as ban lists and leaderboards.

When starting the game, the player selects a scenario, difficulty level, and a character. Each difficulty level changes the enemies and items the player encounters as they progress. The game has five scenarios: "Wild Things", "Underbelly", "Flashback", "Desperate Times" and "End of the Road". There are two bonus scenarios, "Elimination" and "Showdown", designed as training guides to improve gameplay. Each scenario has an event checklist consisting of special actions that the player must perform to reach 100% completion. At 100%, the player unlocks "Infinity Mode", in which the player's weapons never break or run out of ammunition. Completing the game in offline unlocks the option to choose one AI partner instead of two or no AI partners while replaying the five scenarios.

Each scenario also has invisible, randomly generated special or "SP" items hidden throughout the level. There are twenty SP items for each scenario, and twenty items specific to each character hidden across the five scenarios. If acquired, these items unlock new costumes and the option to listen to character ad-libs.

If players wished to stop playing, they could save their data and restart from the scenario they were up to at the time. Players can also save their progress using typewriters in offline.

The game begins with up to four players in online or one player with two AI partners in offline. The player characters are infected with the T-virus, which is shown as a virus gauge, via the inventory menu. Players must complete each scenario before the virus gauge reaches 100% or they will die, resulting in a game over. When players take too much damage, they will collapse and crawl, prompting the virus to speed up faster until the player uses herbs, first aid sprays or recovery medicines on themselves, or another player/AI partner helps them back up on their feet to slow down the virus. If the player succumbs to the virus in online, they will transform into a zombie and continue to control their character by attacking the other players until the mutated player is defeated or dies instantly after a certain amount of time has passed. The player and their AI partners will not transform in offline. Exclusively in File #2, the AI partners will randomly offer items to the player from time to time and are able to carry the injured player around.

The sequel features crossover data where costumes and skins that were purchased and stored in a save file can be transferred to Outbreak or File #2.