Ruth Benerito began her career at the USDA's Southern Regional Research Center (SRRC) in New Orleans. This marked the beginning of her decades-long dedication to textile research.

Benerito conducted extensive research on modifying cotton fibers to make them wrinkle-resistant, stain-resistant, and flame-retardant. Her work focused on cross-linking cellulose molecules, a process that improved the performance of cotton fabrics.

Benerito's most notable achievement was her contribution to the development of wash-and-wear cotton fabrics. Her research on cross-linking enabled cotton garments to resist wrinkling after washing, revolutionizing the textile industry and consumer experience.

Benerito obtained numerous patents for her inventions related to textile chemistry, including methods for treating cotton fabrics and improving their properties. These patents reflected the innovative nature of her research.

Benerito held various leadership positions at the SRRC, including serving as a research leader and mentor to other scientists. She played a key role in shaping the research direction of the center.

Ruth Benerito retired from the USDA SRRC after a distinguished career spanning several decades. However, she remained active in the scientific community and continued to consult on textile-related issues.