Scholastica (/skəˈlæstɪkə/; c. 480 – 10 February 543) was an Italian Christian hermit and the sister of Benedict of Nursia. She is traditionally regarded as the foundress of the Benedictine nuns.

Scholastica is honored as a saint of the Catholic Church, Eastern Orthodox Church and Anglican Communion. She was born in Italy, and a ninth-century tradition makes her the twin sister of Benedict. Her feast day is 10 February.

According to the 6th-century Dialogues of Gregory the Great, Scholastica was born c. 480 in Nursia, Umbria, of wealthy parents (Anicius Eupropius and his wife Claudia Abondantia Reguardati). While Gregory only states that Scholastica was Benedict's sister, a later tradition says she was his twin (whether this is meant biologically or spiritually, or both, is unclear). Gregory also says she was dedicated to God from a young age. She and her brother Benedict were brought up together until the time he left to pursue studies in Rome.

A young Roman woman of Scholastica's class and time would likely have remained in her father's house until marriage or entry into consecrated life. On occasion several consecrated virgins would live together in a household and form a community.

Benedictine history shows that Scholastica established a hermitage about five miles from Monte Cassino and that this was the first convent of Benedictine nuns. However, it is possible that Scholastica lived in a hermitage with one or two other consecrated virgins in a cluster of houses at the base of Mount Cassino where there is an ancient church under her patronage Monastero di Santa Scolastica. Ruth Clifford Engs notes that since Dialogues indicates that Scholastica was dedicated to God at an early age, perhaps she lived in her father's house with other religious women until his death and then moved nearer to Benedict.

The most commonly told story about her is that Scholastica would, once a year, go and visit her brother at a place near his abbey, and they would spend the day worshiping together and discussing sacred texts and issues.

One day they had supper and continued their conversation. When Benedict indicated it was time for him to leave, Scholastica, perhaps sensing that the time of her death was drawing near, asked him to stay with her for the evening so they could continue their discussions. Not wishing to break his own Rule, Benedict refused, insisting that he needed to return to his cell. At that point, Scholastica closed her hands in prayer, and after a moment, a wild storm started outside of the guest house in which they were staying. Benedict asked, "What have you done?", to which she replied, "I asked you and you would not listen; so I asked my God and he did listen. So now go off, if you can; leave me and return to your monastery." Benedict was unable to return to his monastery, and they spent the night in discussion.

Three days later, from his cell, he saw his sister's soul leaving the earth and ascending to heaven in the form of a shining white dove. Benedict had her body brought to his monastery, where he had it laid in the tomb which he had prepared for himself.