The Slaughter-House Cases, 83 U.S. (16 Wall.) 36 (1873), was a landmark U.S. Supreme Court decision which ruled that the Privileges or Immunities Clause of the Fourteenth Amendment to the U.S. Constitution only protects the legal rights that are associated with federal U.S. citizenship, not those that pertain to state citizenship. Though the decision in the Slaughter-House Cases minimized the impact of the Privileges or Immunities Clause on state law, the Supreme Court would later incorporate the Bill of Rights to strike down state laws on the basis of other clauses. In 2010, the Court rejected arguments in McDonald v. Chicago to overrule the established precedent of Slaughterhouse and decided instead to incorporate the Second Amendment via the Due Process Clause of the Fourteenth Amendment.

Ostensibly seeking to improve sanitary conditions, the Louisiana legislature and the city of New Orleans had established a corporation charged with regulating the slaughterhouse industry. Members of the Butchers' Benevolent Association challenged the constitutionality of the corporation's monopoly, claiming that it violated the Fourteenth Amendment. The amendment had been ratified in the aftermath of the American Civil War with the primary intention of protecting civil rights of millions of newly emancipated freedmen in the Southern United States, but the butchers argued that the amendment protected their right to "sustain their lives through labor".

In the majority opinion written by Associate Justice Samuel Freeman Miller, the Court held to a narrower interpretation of the Fourteenth Amendment than the plaintiffs urged, ruling that it did not restrict the police powers exercised by Louisiana because the Privileges or Immunities Clause protected only those rights guaranteed by the United States, not individual states. In effect, the clause was interpreted to convey limited protection pertinent to a small minority of rights, such as the right to seek federal office.

In a dissenting opinion, Associate Justice Stephen J. Field wrote that Miller's opinion effectively rendered the Fourteenth Amendment a "vain and idle enactment".

One writer described New Orleans in the mid-nineteenth century as plagued by "intestines and portions of putrefied animal matter lodged [around the drinking pipes]" whenever the tide from the Mississippi River was low; the offal came from the city's slaughterhouses. A mile and a half upstream from the city, 1,000 butchers gutted more than 300,000 animals per year. Animal entrails (known as offal), dung, blood, and urine contaminated New Orleans's drinking water, which was implicated in cholera and yellow fever outbreaks among the population.

To try to control the problem, a New Orleans grand jury recommended that the slaughterhouses be moved south, but since many of the slaughterhouses were outside city limits, the grand jury's recommendations carried no weight. The city appealed to the state legislature. As a result, in 1869, the Louisiana legislature passed "An Act to Protect the Health of the City of New Orleans, to Locate the Stock Landings and Slaughter Houses, and to incorporate the Crescent City Livestock Landing and Slaughter-House Company", which allowed the city of New Orleans to create a corporation that centralized all slaughterhouse operations in the city. At the time, New York City, San Francisco, Boston, Milwaukee, and Philadelphia had similar provisions to confine butchers' establishments to particular areas in order to keep offal from contaminating the water supply.

The legislature chartered a private corporation, the Crescent City Live-Stock Landing and Slaughter-House Company, to run a Grand Slaughterhouse at the southern part of the city, opposite the Mississippi River. Crescent City would not slaughter beef itself but act as a franchise corporation, by renting out space to other butchers in the city for a fee, under a designated maximum.

The statute also granted "sole and exclusive privilege of conducting and carrying on the livestock landing and slaughterhouse business within the limits and privilege granted by the act, and that all such animals shall be landed at the stock landings and slaughtered at the slaughterhouses of the company, and nowhere else. Penalties are enacted for infractions of this provision, and prices fixed for the maximum charges of the company for each steamboat and for each animal landed". The exclusivity would last for a period of 25 years. All other slaughterhouses would be closed up, forcing butchers to slaughter within the operation set up by Crescent City. The statute forbade Crescent City from favoring one butcher over another by promising harsh penalties for refusal to sell space to any butcher. All animals on the premises would be inspected by an officer appointed by the governor of the state.