Astrobiology (also xenology or exobiology) is a scientific field within the life and environmental sciences that studies the origins, early evolution, distribution, and future of life in the universe by investigating its deterministic conditions and contingent events. As a discipline, astrobiology is founded on the premise that life may exist beyond Earth.

Research in astrobiology comprises three main areas: the study of habitable environments in the Solar System and beyond, the search for planetary biosignatures of past or present extraterrestrial life, and the study of the origin and early evolution of life on Earth.

The field of astrobiology has its origins in the 20th century with the advent of space exploration and the discovery of exoplanets. Early astrobiology research focused on the search for extraterrestrial life and the study of the potential for life to exist on other planets. In the 1960s and 1970s, NASA began its astrobiology pursuits within the Viking program, which was the first US mission to land on Mars and search for signs of life. This mission, along with other early space exploration missions, laid the foundation for the development of astrobiology as a discipline.

Regarding habitable environments, astrobiology investigates potential locations beyond Earth that could support life, such as Mars, Europa, and exoplanets, through research into the extremophiles populating austere environments on Earth, like volcanic and deep sea environments. Research within this topic is conducted utilising the methodology of the geosciences, especially geobiology, for astrobiological applications.

The search for biosignatures involves the identification of signs of past or present life in the form of organic compounds, isotopic ratios, or microbial fossils. Research within this topic is conducted utilising the methodology of planetary and environmental science, especially atmospheric science, for astrobiological applications, and is often conducted through remote sensing and in situ missions.

Astrobiology also concerns the study of the origin and early evolution of life on Earth to try to understand the conditions that are necessary for life to form on other planets. This research seeks to understand how life emerged from non-living matter and how it evolved to become the diverse array of organisms we see today. Research within this topic is conducted utilising the methodology of paleosciences, especially paleobiology, for astrobiological applications.

Astrobiology is a rapidly developing field with a strong interdisciplinary aspect that holds many challenges and opportunities for scientists. Astrobiology programs and research centres are present in many universities and research institutions around the world, and space agencies like NASA and ESA have dedicated departments and programs for astrobiology research.

The term astrobiology was first proposed by the Russian astronomer Gavriil Tikhov in 1953. It is etymologically derived from the Greek ἄστρον, "star"; βίος, "life"; and -λογία, -logia, "study". A close synonym is exobiology from the Greek Έξω, "external"; βίος, "life"; and -λογία, -logia, "study", coined by American molecular biologist Joshua Lederberg; exobiology is considered to have a narrow scope limited to search of life external to Earth. Another associated term is xenobiology, from the Greek ξένος, "foreign"; βίος, "life"; and -λογία, "study", coined by American science fiction writer Robert Heinlein in his 1954 work The Star Beast; xenobiology is now used in a more specialised sense, referring to 'biology based on foreign chemistry', whether of extraterrestrial or terrestrial (typically synthetic) origin.