Tsuyoshi Otsuki (大槻 毅, Otsuki Tsuyoshi; born December 1, 1972) is a former Japanese football player and manager. He was most recently manager of J2 League club Thespa Gunma.

Otsuki was born in Sendai on December 1, 1972. After graduating from University of Tsukuba, he played for his local club Sony Sendai.

After retiring players career, Otsuki became J.League club coach. (Mito HollyHock (2000-2002), Omiya Ardija (2003), Urawa Reds (2004-2010) and Vegalta Sendai (2011)) In 2012, he returned Urawa Reds. On April 2, 2018, team manager Takafumi Hori was sacked. Otsuki became manager. On April 22, the club contracted with Oswaldo de Oliveira. So, Otsuki became assistant coach. In May 2019, manager Oliveira was sacked and Otsuki became a manager again.

On 16 December 2021, Otsuki announcement officially appointment manager of Thespakusatsu Gunma (now Thespa Gunma) from 2022 season.

Following a poor start to the 2024 season with 10 losses in their first 14 games, Otsuki was dismissed in May 2024.