Wat Bot (Thai: วัดโบสถ์, pronounced [wát bòːt]) is a district (amphoe) in the northern part of Phitsanulok province, lower northern region of Thailand.

Tambon Wat Bot was separated from the Phrom Phiram district and created as a minor district (king amphoe) on 1 January 1948. It was upgraded to a full district on 6 June 1956. The present district office was opened on 4 July 1991.

Neighboring districts are (from the east clockwise), Chat Trakan, Wang Thong, Mueang Phitsanulok, and Phrom Phiram of Phitsanulok Province; Phichai and Thong Saen Khan of Uttaradit province.

Wat Bot lies within the Nan Basin, which is part of the Chao Phraya Watershed. The Khwae Noi River flows through Wat Bot District.

Portions of Wat Bot are part of the Khwae Noi National Reserved Forest, which was recently made part of Kaeng Chet Khwae National Park.

The district is divided into six subdistricts (tambons), which are further subdivided into 61 villages (mubans), as of 2025: 36,633 people of 16,103 families.

There is one subdistrict municipality (thesaban tambon}. Wat Bot municipality covers parts of villages 1 - 4, 7, 8 of Wat Bot, parts of villages 3, 4, 11, 12 of Tha Ngam, parts of 2 - 4 of Thothae subdistricts.

Further there are six subdistrict administrative organizations (SAO). Wat Bot, Thothae and Tha Ngam SAO's cover the remaining areas of Wat Bot, Thothae and Tha Ngam subdistricts. So the other three SAO's cover the whole same-named subdistrict.