Wuzu Temple (Chinese: 五祖寺; pinyin: Wǔzǔ Sì; lit. 'Temple of the 5th Patriarch') is a Buddhist temple located on the Eastern Mountain, in Wuzu Town of Huangmei County, Hubei, China.

Wuzu Temple was first built in 654 with the name of Dongshan Temple (东山寺; 'Eastern Mountain Temple'). During the Tang dynasty (618–907), Abbot Daman Hongren, an eminent monk of Chan Buddhism, promulgated Buddhist doctrines for decades. His disciples includes Huineng, Shenxiu, and Faru.

In 848, Emperor Xuanzong named it "Dazhong Dongshan Temple" (大中东山寺; 'Great Central Eastern Mountain Temple').

In the Song dynasty (960–1279), Shijie (师戒), Fayan (法演), Biaozi (表自) and Zongba (宗拔) successively served as abbot of the temple. While Fayan settled at the temple, it had reached unprecedented heyday, his disciples Yuanwu Keqin, Fojian Huiqin and Foyan Qingyuan were hailed as "Three luminaries" or "Three Buddhas".

During the Jingde period (1004–1007), Emperor Zhenzong inscribed and honored the name of "Zhen Hui Chan Temple" (真慧禅寺).

After the fall of the Song dynasty, Wuzu Temple was completely destroyed by fire during the Mongolian invasion of the 13th century.

In 1282, at the dawn of the Yuan dynasty (1271–1368), master Liaoxing (了行) restored and redecorated the temple.

In 1313, the mummy of Daman Hongren was brought back and enshrined in the temple.