| XIV Venice Challenge Save Cup | |
|---|---|
| Date | 16–22 May |
| Edition | 3rd |
| Draw | 32S / 16D |
| Prize money | €42,500 |
| Surface | Clay |
| Location | Mestre, Italy |
| Champions | |
| Singles | |
 Gastão Elias | |
| Doubles | |
 Fabrício Neis / Caio Zampieri | |
The XIV Venice Challenge Save Cup was a professional tennis tournament played on clay courts. It was the 3rd edition of the men's tournament which was part of the 2016 ATP Challenger Tour. It took place in Mestre, Italy between 16 and 22 May 2016.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
 ITA
|
Paolo Lorenzi | 53 | 1 |
 SRB
|
Dušan Lajović | 70 | 2 |
 ARG
|
Horacio Zeballos | 90 | 3 |
| POR
|
Gastão Elias | 92 | 4 |
 FRA
|
Constant Lestienne | 210 | 5 |
| POR
|
Frederico Ferreira Silva | 243 | 6 |
 CAN
|
Steven Diez | 247 | 7 |
| ITA
|
Salvatore Caruso | 251 | 8 |
The following players received wildcards into the singles main draw:
Edoardo Eremin Gianluca Mager Stefano Napolitano Lorenzo SonegoThe following player entered the main draw as an alternate:
The following players received entry from the qualifying draw:
Gastão Elias def. Horacio Zeballos, 7–6(7–0), 6–2 Fabrício Neis / Caio Zampieri def. 
Kevin Krawietz / 
Dino Marcan, 7–6(7–3), 4–6, [12–10]
