The 2000 National Lacrosse League season is the 14th season in the NLL that began on January 7, 2000, and concluded with the championship game on May 6, 2000. The Toronto Rock defeated the Rochester Knighthawks 14–13. Kaleb Toth scored the winning goal with less than two seconds left in regulation to give the Rock their second straight championship.

One expansion team was added to the NLL for the 2000 season, the Albany Attack. In addition, the Baltimore Thunder left Baltimore and became the Pittsburgh CrosseFire, though the franchise would only last a single season in Pittsburgh before moving to Washington.

Reference:

x: Clinched playoff berth; c: Clinched playoff berth by crossing over to another division; y: Clinched division; z: Clinched best regular season record; GP: Games Played
W: Wins; L: Losses; GB: Games back; PCT: Win percentage; Home: Record at Home; Road: Record on the Road; GF: Goals scored; GA: Goals allowed
Differential: Difference between goals scored and allowed; GF/GP: Average number of goals scored per game; GA/GP: Average number of goals allowed per game

No All-Star game was held in 2000.

Each week, a player is awarded "Player of the Week" honours.

Awards are also given out monthly for the best overall player and best rookie.

Bold numbers indicate new single-season records. Italics indicate tied single-season records.