Anna Maria Marconi is a fictional character appearing in American comic books published by Marvel Comics. She was initially depicted as a love interest of Spider-Man, notably during the time when his body was taken over by Doctor Octopus's mind. She has since been depicted as a close friend to Peter Parker after he regains his mind.

Anna Maria Marconi first appeared in The Superior Spider-Man #5 (May 2013) and was created by Dan Slott and Giuseppe Camuncoli.

Marconi was created in part from Slott's desire to introduce a character that could cause some friction in Peter's personal life. He found this difficult to pull off as he could not find a reason why a character like Betty Brant or Aunt May could be bigoted or racist. Rather than having any one of them have such a fierce trait, he had May's opinion of Peter dating Anna Maria be that of concern:

Having Aunt May not be perfect is interesting. And when Aunt May is talking about this, it doesn't sound like a prejudice. It's a concerned grandma, wondering what her grandchildren are going to be like, and being ignorant. Aunt May did have cause to dislike the woman...Anna Maria does the worst possible thing you can do to Aunt May, which is to serve wheat cakes. She uses Aunt May's recipe and improves on them.

He also found it relieving that Peter's mind was taken over by Otto Octavius, as he felt that the character was more open-minded than Peter:

With Anna Maria, it was fun to have this character who never knew Peter. And you quickly see that the things that she likes are Otto...You look at Otto, and he really sees people for who they are inside, and everyone he has chosen to love and be with, there's something very neat about that.

Anna Maria Marconi is a young woman with dwarfism who attends Empire State University. At the time when his mind was in Peter Parker's body, Doctor Octopus visits her to become a physics teacher and earn his doctorate. The two share great admiration for each other and the next day, Otto pulls a brutal prank on a group of bullies. The two set up a date together that ends with Otto saving Anna Maria from dry ice and kissing her. However, she too falls under the same worry umbrella as many of Peter's friends and family due to his frequent absences. Later, Anna Maria accepts Otto's offer to join Parker Industries and afterwards, successfully helps him earn his doctorate.

Anna Maria eventually meets Aunt May who despite her friendliness, causes some concern when she asks about her height. She later confides in Otto that she feels that their evening together was her fault because she had hurt the relationship between him and May. During the events of Goblin Nation, Anna Maria was kidnapped by the Menace who had deduced that Peter was really Otto. Norman Osborn as the Goblin King held her hostage to lure his Superior Spider-Man form out. However, Otto had given up his mind and let Peter take back control of Spider-Man and he successfully rescued Anna Maria. She reveals to Peter that she had deduced that he was Spider-Man and that he planned on proposing to her, but is shocked to learn that Otto Octavius was the one she was in love with. After baking him cookies, she leaves to clear her head. She soon meets back up with Peter at Parker Industries to help him with some of Otto's work and continues to help Peter on a platonic basis.