Artificial gravity is the creation of an inertial force that mimics the effects of a gravitational force, usually by rotation. Artificial gravity, or rotational gravity, is thus the appearance of a centrifugal force in a rotating frame of reference (the transmission of centripetal acceleration via normal force in the non-rotating frame of reference), as opposed to the force experienced in linear acceleration, which by the equivalence principle is indistinguishable from gravity. In a more general sense, "artificial gravity" may also refer to the effect of linear acceleration, e.g. by means of a rocket engine.

Rotational simulated gravity has been used in simulations to help astronauts train for extreme conditions. Rotational simulated gravity has been proposed as a solution in human spaceflight to the adverse health effects caused by prolonged weightlessness. However, there are no current practical outer space applications of artificial gravity for humans due to concerns about the size and cost of a spacecraft necessary to produce a useful centripetal force comparable to the gravitational field strength on Earth (g). Scientists are concerned about the effect of such a system on the inner ear of the occupants. The concern is that using centripetal force to create artificial gravity will cause disturbances in the inner ear leading to nausea and disorientation. The adverse effects may prove intolerable for the occupants.

In the context of a rotating space station, it is the radial force provided by the spacecraft's hull that acts as centripetal force. Thus, the "gravity" force felt by an object is the centrifugal force perceived in the rotating frame of reference as pointing "downwards" towards the hull.

By Newton's third law, the value of little g (the perceived "downward" acceleration) is equal in magnitude and opposite in direction to the centripetal acceleration. It was tested with satellites like Bion 3 (1975) and Bion 4 (1977); they both had centrifuges on board to put some specimens in an artificial gravity environment.

From the perspective of people rotating with the habitat, artificial gravity by rotation behaves similarly to normal gravity but with the following differences, which can be mitigated by increasing the radius of a space station.

The Gemini 11 mission attempted in 1966 to produce artificial gravity by rotating the capsule around the Agena Target Vehicle to which it was attached by a 36-meter tether. They were able to generate a small amount of artificial gravity, about 0.00015 g, by firing their side thrusters to slowly rotate the combined craft like a slow-motion pair of bolas. The resultant force was too small to be felt by either astronaut, but objects were observed moving towards the "floor" of the capsule.

Artificial gravity has been suggested as a solution to various health risks associated with spaceflight. In 1964, the Soviet space program believed that a human could not survive more than 14 days in space for fear that the heart and blood vessels would be unable to adapt to the weightless conditions. This fear was eventually discovered to be unfounded as spaceflights have now lasted up to 437 consecutive days, with missions aboard the International Space Station commonly lasting 6 months. However, the question of human safety in space did launch an investigation into the physical effects of prolonged exposure to weightlessness. In June 1991, the Spacelab Life Sciences 1 on the Space Shuttle flight STS-40 flight performed 18 experiments on two men and two women over nine days. In an environment without gravity, it was concluded that the response of white blood cells and muscle mass decreased. Additionally, within the first 24 hours spent in a weightless environment, blood volume decreased by 10%. Long periods of weightlessness can cause brain swelling and eyesight problems. Upon return to Earth, the effects of prolonged weightlessness continue to affect the human body as fluids pool back to the lower body, the heart rate rises, a drop in blood pressure occurs, and there is a reduced tolerance for exercise.

Artificial gravity, for its ability to mimic the behavior of gravity on the human body, has been suggested as one of the most encompassing manners of combating the physical effects inherent in weightless environments. Other measures that have been suggested as symptomatic treatments include exercise, diet, and Pingvin suits. However, criticism of those methods lies in the fact that they do not fully eliminate health problems and require a variety of solutions to address all issues. Artificial gravity, in contrast, would remove the weightlessness inherent in space travel. By implementing artificial gravity, space travelers would never have to experience weightlessness or the associated side effects. Especially in a modern-day six-month journey to Mars, exposure to artificial gravity is suggested in either a continuous or intermittent form to prevent extreme debilitation to the astronauts during travel.