The Asiatic cheetah (Acinonyx jubatus venaticus) is a critically endangered cheetah subspecies currently surviving in Iran. Its range once spread from the Arabian Peninsula and the Near East to the Caspian region, Transcaucasus, Kyzylkum Desert and northern South Asia, but was extirpated in these regions during the 20th century. The Asiatic cheetah diverged from the cheetah population in Africa between 32,000 and 67,000 years ago.

The Asiatic cheetah survives in protected areas in the eastern-central arid region of Iran. Between December 2011 and November 2013, 84 individuals were sighted in 14 different protected areas, and 82 individuals were identified from camera trap photographs. In December 2017, fewer than 50 individuals were thought to be remaining in three subpopulations that are scattered over 140,000 km² (54,000 sq mi) in Iran's central plateau.

Felis venatica was proposed by Edward Griffith in 1821 and based on a sketch of a maneless cheetah from India. Griffith's description was published in Le Règne Animal with the help of Griffith's assistant Charles Hamilton Smith in 1827.

Acinonyx raddei was proposed by Max Hilzheimer in 1913 for the cheetah population in Central Asia, the Trans-Caspian cheetah. Hilzheimer's type specimen originated in Merv, Turkmenistan.

Results of a five-year phylogeographic study on cheetah subspecies indicate that Asiatic and African cheetah populations separated between 32,000 and 67,000 years ago and are genetically distinct. Samples of 94 cheetahs for extracting mitochondrial DNA were collected in nine countries from wild, seized and captive individuals and from museum specimen. The population in Iran is considered autochthonous monophyletic and the last remaining representative of the Asiatic subspecies. Mitochondrial DNA fragments of an Indian and a Southeast African cheetah museum specimens showed that they genetically diverged about 72,000 years ago.

The Asiatic cheetah has a buff-to-light fawn-coloured fur that is paler on the sides, on the front of the muzzle, below the eyes and inner legs. Small black spots are arranged in lines on the head and nape, but irregularly scattered on body, legs, paws and tail. The tail tip has black stripes. The coat and mane are shorter than of African cheetah subspecies. The head and body of an adult Asiatic cheetah measure about 112–135 cm (44–53 in) with a 66–84 cm (26–33 in) long tail. It weighs about 34–54 kg (75–119 lb). It exhibits sexual dimorphism; males are slightly larger than the females.

The cheetah is the fastest land animal in the world. It was previously thought that the body temperature of a cheetah increases during a hunt due to high metabolic activity. In a short period of time during a chase, a cheetah may produce 60 times more heat than at rest, with much of the heat, produced from glycolysis, stored to possibly raise the body temperature. The claim was supported by data from experiments in which two cheetahs ran on a treadmill for minutes on end but contradicted by studies in natural settings, which indicate that body temperature stays relatively the same during a hunt. A 2013 study suggested stress hyperthermia and a slight increase in body temperature after a hunt. The cheetah's nervousness after a hunt may induce stress hyperthermia, which involves high sympathetic nervous activity and raises the body temperature. After a hunt, the risk of another predator taking its kill is great, and the cheetah is on high alert and stressed. The increased sympathetic activity prepares the cheetah's body to run when another predator approaches. In the 2013 study, even the cheetah that did not chase the prey experienced an increase in body temperature once the prey was caught, showing increased sympathetic activity.

The cheetah thrives in open lands, small plains, semi-desert areas, and other open habitats where prey is available. The Asiatic cheetah mainly inhabits the desert areas around Dasht-e Kavir in the eastern half of Iran, including parts of the Kerman, Khorasan, Semnan, Yazd, Tehran and Markazi provinces. Most live in five protected areas, viz Kavir National Park, Khar Turan National Park, Bafq Protected Area, Dar-e Anjir Wildlife Refuge, and Naybandan Wildlife Reserve.