The Bendigo line is a regional passenger rail service operated by V/Line in Victoria, Australia. It serves 14 stations towards its terminus in the regional city of Bendigo. It is the third most-used regional rail service in Victoria (behind the Ballarat and Geelong services), carrying 2.06 million passengers in the 2022-23 financial year.

Beyond Bendigo, the Echuca line continues north-east to Echuca via Epsom; and the Swan Hill line continues north-west to Swan Hill via Eaglehawk. Services are operated to Bendigo and Echuca primarily using V/Line VLocity diesel multiple unit sets in either 3- or 6-car configurations; V/Line N class loco-hauled services run to Swan Hill, with a mixture of N type carriages and Z type carriages.

In the 1850s, the Melbourne, Mount Alexander and Murray River Railway Company began building a line between Melbourne to Bendigo, but ran into financial difficulties and, in 1856, was sold to the Victorian government, which finished the double-track line in 1862. Throughout the early 20th century, many branch lines were built along the length of the Bendigo line, but by the time V/Line took over Victorian regional passenger services in 1983, the only passengers services still using the Bendigo corridor were the Bendigo and Swan Hill intercity services. A twice-weekly passenger service to Echuca from Melbourne via Bendigo was reinstated in 1996.

As part of the Regional Fast Rail project, which was completed in 2006, the Bendigo line underwent major track upgrades that would allow for V/Line VLocity diesel multiple unit trains to be introduced to the line, running at speeds of up to 160km/h between Sunbury and Bendigo. Controversially, between Kyneton and Bendigo the line was singled, officially to allow for better clearance past heritage structures, although some double-track sections were retained, effectively creating long crossing loops. Those sections of track were provided with bi-directional signalling, but only the main running line was upgraded for high-speed running.

In 2012, electrification on the line was extended from Sydenham (now Watergardens) to Sunbury along the V/Line tracks. This caused some controversy among frequent users of V/Line's Bendigo services who claimed it would cause extra congestion, slowing down the Bendigo service. Ian Dobbs, then head of Public Transport Victoria acknowledged this issue, stating that "there's a slight slow-down of services on that particular corridor with this timetable, because we're putting more Metro services in the mix."

In 2008, the Regional Rail Link project was announced. Finished in 2015, it consists of a new pair of tracks exclusively for V/Line services, avoiding the need to share tracks with Metro's electrified suburban services. The tracks begin west of Werribee, following an alignment up to Deer Park, then running into two new platforms at Southern Cross, completely bypassing Tottenham, West Footscray, Middle Footscray, South Kensington, and North Melbourne; but still including stops at Sunshine and Footscray at two platforms dedicated to V/Line services. All current timetabled Bendigo services run express through Sunshine.

The main benefit of the project was for the Geelong line service, which used to run along the same tracks as Metro's Werribee line, resulting in significant congestion. Ballarat and especially Bendigo services benefited less, only being separated from Metro's Sunbury line service between Sunshine and Southern Cross. While the project was overall well-received for increasing service reliability, the choice to bypass North Melbourne was especially criticised.

In 2014, Epsom Station was constructed to serve Bendigo's northern suburb of the same name. It was initially serviced four times daily by extending some services that terminated at Bendigo. In 2016, three services that would have terminated at Bendigo were extended to Eaglehawk Station, and all weekday services to Bendigo began to stop at Kangaroo Flat.