In broadcasting and radio communications, a call sign (also known as a call name or call letters—and historically as a call signal—or abbreviated as a call) is a unique identifier for a transmitter station. A call sign can be formally assigned by a government agency, informally adopted by individuals or organizations, or even cryptographically encoded to disguise a station's identity.

The use of call signs as unique identifiers dates to the landline railroad telegraph system. Because there was only one telegraph line linking all railroad stations, there needed to be a way to address each one when sending a telegram. In order to save time, two-letter identifiers were adopted for this purpose. This pattern continued in radiotelegraph operation; radio companies initially assigned two-letter identifiers to coastal stations and stations on board ships at sea. These were not globally unique, so a one-letter company identifier (for instance, 'M' and two letters as a Marconi station) was later added. By 1912, the need to quickly identify stations operated by multiple companies in multiple nations required an international standard; an ITU prefix would be used to identify a country, and the rest of the call sign an individual station in that country.

Merchant and naval vessels are assigned call signs by their national licensing authorities. In the case of states such as Liberia or Panama, which are flags of convenience for ship registration, call signs for larger vessels consist of the national prefix plus three letters (for example, 3LXY, and sometimes followed by a number, e.g. 3LXY2). United States merchant vessels are given call signs beginning with the letters "W" or "K" while US naval ships are assigned call signs beginning with "N". Originally, both ships and broadcast stations were assigned call signs in this series consisting of three or four letters. Ships equipped with Morse code radiotelegraphy, or life boat radio sets, aviation ground stations, broadcast stations were given four-letter call signs. Maritime coast stations on high frequency (both radiotelegraphy and radiotelephony) were assigned three-letter call signs. As demand for both marine radio and broadcast call signs grew, gradually American-flagged vessels with radiotelephony only were given longer call signs with mixed letters and numbers.

Leisure craft with VHF radios may not be assigned call signs, in which case the name of the vessel is used instead. Ships in the US still wishing to have a radio license are under FCC class SA: "Ship recreational or voluntarily equipped." Those calls follow the land mobile format of the initial letter K or W followed by one or two letters followed by three or four numbers (such as KX0983 or WXX0029). U.S. Coast Guard small boats have a number that is shown on both bows (i.e. port and starboard) in which the first two digits indicate the nominal length of the boat in feet. For example, Coast Guard 47021 refers to the 21st in the series of 47-foot motor lifeboats. The call sign might be abbreviated to the final two or three numbers during operations, for example: Coast Guard zero two one.

Originally aviation mobile stations (aircraft) equipped with radiotelegraphy were assigned five-letter call signs (e.g. KHAAQ). Land stations in aviation were assigned four-letter call signs (e.g. WEAL – Eastern Air Lines, NYC.) These call signs were phased out in the 1960s when flight radio officers (FRO) were no longer required on international flights. The Russian Federation kept FROs for the Moscow-Havana run until around 2000.

Currently, all signs in aviation are derived from several different policies, depending upon the type of flight operation and whether or not the caller is in an aircraft or at a ground facility. In most countries, unscheduled general aviation flights identify themselves using the call sign corresponding to the aircraft's registration number (also called N-number in the U.S., or tail number). In this case, the call sign is spoken using the International Civil Aviation Organization (ICAO) phonetic alphabet. Aircraft registration numbers internationally follow the pattern of a country prefix, followed by a unique identifier made up of letters and numbers. For example, an aircraft registered as N978CP conducting a general aviation flight would use the call sign November-niner-seven-eight-Charlie-Papa. However, in the United States a pilot of an aircraft would normally omit saying November, and instead use the name of the aircraft manufacturer or the specific model. At times, general aviation pilots might omit additional preceding numbers and use only the last three numbers and letters. This is especially true at uncontrolled fields (those without control towers) when reporting traffic pattern positions or at towered airports after establishing two-way communication with the tower controller. For example, Skyhawk eight-Charlie-Papa, left base. In commercial aviation, the callsign is usually the ICAO Flight number. For example, Delta Air Lines Flight 744 would have the flight number DL744 and the callsign would be Delta 744.

In most countries, the aircraft call sign or "tail number"/"tail letters" (also known as registration marks) are linked to the international radio call sign allocation table and follow a convention that aircraft radio stations (and, by extension, the aircraft itself) receive call signs consisting of five letters. For example, all British civil aircraft have a five-letter registration beginning with the letter G, which can also serve for a call sign. Canadian aircraft have a call sign beginning with C–F or C–G, such as C–FABC. wing-in-ground-effect vehicles and hovercraft in Canada are eligible to receive C–Hxxx call signs, and ultralight aircraft receive C-Ixxx call signs. In days gone by, even American aircraft used five-letter call signs, such as KH–ABC, but they were replaced prior to World War II by the current American system of civilian aircraft call signs (see below). One exception to the parallelism between registration and call sign is ultralight airplanes in France, who are not obliged to carry a radio and indeed often don't.

Radio call signs used for communication in crewed spaceflight are not formalized or regulated to the same degree as for aircraft. The three nations currently launching crewed space missions use different methods to identify the ground and space radio stations; the United States uses either the names given to the space vehicles, or else the project name and mission number. Russia traditionally assigns code names as call signs to individual cosmonauts, more in the manner of aviator call signs, rather than to the spacecraft.