Calvary Cemetery is a Catholic cemetery in Maspeth and Woodside, Queens, in New York City, New York, United States. With about three million burials, it has the largest number of interments of any cemetery in the United States. Established in 1848, Calvary Cemetery covers 365 acres (148 ha) and is owned by the Archdiocese of New York and managed by the Trustees of St. Patrick's Cathedral.

Calvary Cemetery is divided into four sections, spread across the neighborhoods of Maspeth and Woodside. The oldest, First Calvary, is also called "Old Calvary". The Second, Third and Fourth sections are all considered part of "New Calvary".

In 1817, the Trustees of Old St. Patrick's Cathedral on Mott Street, Manhattan, realized that their original cemetery on Mulberry Street was almost full. In 1847, faced with cholera epidemics and a shortage of burial grounds in Manhattan, the New York State Legislature passed the Rural Cemetery Act authorizing nonprofit corporations to operate commercial cemeteries. On October 29, 1845 Old St. Patrick's Cathedral trustees had purchased 71 acres (29 ha) of land from John McMenoy and John McNolte in Maspeth and this land was used to develop Calvary Cemetery. The cemetery was named after Mount Calvary, where Jesus Christ was crucified according to the New Testament.

The first Calvary Cemetery burial occurred on July 31, 1848. The name of the deceased was Esther Ennis, who reportedly "died of a broken heart." The cemetery was consecrated by Archbishop John Hughes in August 1848.

By 1852 there were 50 burials a day, half of them poor Irish under seven years of age. In the early 20th century, influenza and tuberculosis epidemics caused a shortage of gravediggers, and people dug graves for their own loved ones. The entire number of interments from the cemetery's opening in August 1848 until January 1898, was 644,761. From January 1898 until 1907 there were about 200,000 interments, thus yielding roughly 850,000 interments at Calvary Cemetery by 1907.

Calvary was accessible by ferryboats crossing the East River from 23rd Street in Manhattan. It cost an adult seven dollars to be buried there. Burial of children under age seven cost three dollars; children aged seven to fourteen cost five dollars. As development in Manhattan's East Village expanded, bodies buried in that neighborhood were transferred to Queens. In 1854, ferry service opened by 10th Street and the East River.

The original division of the cemetery, now known as First Calvary or Old Calvary, was filled by 1867. The Archdiocese of New York expanded the area of the cemetery, adding more sections, and by the 1990s there were nearly 3 million burials in Calvary Cemetery. The Cemetery continues to add plots and burial spaces can be purchased in advance.

In 1949, several hundred workers at the cemetery went on strike.