Charlton Mackrell is a village and former civil parish, now in the parish of The Charltons, in the Somerset district, in the ceremonial county of Somerset, England, situated 3 miles (5 km) east of Somerton. The village has a population of 1,020.

The parish consists of two villages, Charlton Mackrell and Charlton Adam. Both villages have approximately similar populations. There are also small settlements at Lytes Cary, Cary Fitzpaine (east of the A37 Fosse Way), and West Charlton. West Charlton is the shrunken remains of the original village of Charlton Mackrell.

The Fosse Way runs through the parish, and Roman villa sites associated with it have been found at Windmill Hill. The parishes of Charlton Adam and Charlton Mackrell were part of the hundred of Somerton.

In the 16th century, two medieval fields were divided, which delayed inclosure until the 18th century, leading to the current patchwork of fields.

The Charltons have been home to several of the ancestors of politicians in the United States of America. Henry Adams, of nearby Barton St David, and Edith Squire of Charlton-Mackrell were married in the parish church in 1609, and they were the ancestors of Presidents John Adams, John Quincy Adams, and Calvin Coolidge, as well as of Samuel Adams, who also signed the Declaration of Independence, while Presidents Millard Fillmore and William Howard Taft were descendants of her two sisters Ann and Margaret.

Around 1800, Kingweston resident William Dickinson purchased the manor, and his family owned it until 1930.

A railway station served the village as part of the Great Western Railway from 1905 to 1962.

The village was immortalised in song by Adge Cutler in 1968 with the composition "The Charlton Mackrell Jug Band".