Cycling in Israel refers to both the usage of bicycles for day to day transportation as well as cycling for sport in Israel or by Israelis. Cycling was popular in the coastal regions of Israel in the early 20th century, however following Israel's economic development and modernist car oriented planning, cycling fell out of popular use. The usage of bicycles for transportation has been growing in Israel in the early 21st century while Israel has hosted several cycling competitions as well as produced several renowned cycling champions.

The introduction of bicycles to the region dates back to the late 19th century. Between 1890 and 1893, American students Thomas Gaskell Allen and William Lewis Sachtleben documented their journey across Asia Minor, including parts of the Middle East, on bicycles. Their travels highlighted the rarity of two-wheelers in the Ottoman Empire during that era.

In the early 20th century, prominent Zionist figures, including Theodor Herzl, were known to be cycling enthusiasts. During the British Mandate period the there was a growing adoption of cycling, during this period modern bicycles were first introduced to the region. Jewish sports organizations also promoted cycling as a sport. The Maccabi World Union, established in 1921, and Hapoel, founded in 1926, organized cycling events and competitions. Notably, the first Maccabiah Games in 1932 included cycling competitions, and Hapoel held its first road bike race the same year on the streets of Tel Aviv.

In the early 21st century, cycling has seen a notable increase in popularity across Israel. Between 2012 and 2015, there was a 21% rise in the number of non-motorized two-wheeled vehicles imported into the country. In Tel Aviv, a 2012 survey revealed a 54% increase in bicycle usage since 2010. Approximately 18,000 residents reported using bicycles as their primary mode of transportation to work or school. In 2015, 15% of Tel Avivians cycled for their daily activities such as work. In 2019, Tel Aviv had the highest per capita usage of e-biks. In 2020, it was reported that 10% of Israelis make weekly usage of bikes and E-Bikes. In 2025, the percentage of commuters who cycled back and forth accounted for 17% of Tel Aviv residents.

Yom Kippur, the holiest day of the Jewish calendar, motorized traffic comes to a halt across the country. Israelis, especially secular Israelis go out during the day to cycle, turning city streets into expansive bike paths for riders of all ages.

Jerusalem has 42 km of cycling infrastructure emcompassing the Jerusalem ring project. In 2022, the first cycling tunnel in Jerusalem was opened in Kerem pass. In 2020, Jerusalem advanced a plan to increase the number of bike paths to 120 km.

In the early 20th century, due to Tel Aviv's flat terrain and comfortable weather, cycling became the go to mode of transportation in the city. The economic costs of owning a car also contributed to the usage of cycling.

In the early 1990s, a group of cycling advocates proposed to the Tel Aviv city council the promotion of bicycles as a mode of urban transport. Initially met with skepticism and dismissed as impractical, cycling was seen as incompatible with the city's modern aspirations, with some officials characterizing it as appropriate only for less developed regions. Despite this resistance, persistent efforts by advocates, coupled with the gradual realization of cycling’s potential benefits, led to increased acceptance. By 2015, approximately 15% of central residents utilizied bicycles for commuting. Initiatives such as the introduction of bike lanes and the establishment of the Tel-O-Fun bike-sharing program according to the Guardian have contributed to this cultural shift.