Daisy Duke is a fictional character from the American television series The Dukes of Hazzard. She is the cousin of the protagonists, Bo and Luke, and all three live on a farm on the outskirts of Hazzard County with their Uncle Jesse.

In the television series and the films The Dukes of Hazzard: Reunion! (1997) and The Dukes of Hazzard: Hazzard in Hollywood (2000) she was played by Catherine Bach. In the 2005 film,The Dukes of Hazzard, she was played by Jessica Simpson, and by April Scott in the 2007 prequel film The Dukes of Hazzard: The Beginning

Although never mentioned in the series itself, some press material for the show suggests that Daisy's parents, along with Bo and Luke's, were killed in a car accident; in the 1997 reunion movie, Daisy says her mother died when she was a baby. The series never explained how they all came to live with Uncle Jesse.

Daisy frequently becomes involved in the Dukes' car chases, originally in her Plymouth Road Runner or, from the mid-second season onwards and more famously, in her Jeep. Daisy also works as a waitress at the Boar's Nest, the local tavern owned by Boss Hogg that was the main meeting place in Hazzard. She also aspires to be both a singer-songwriter and a journalist.

"She drives like Richard Petty, shoots like Annie Oakley, and knows the words to all of Dolly Parton's songs."

— The Balladeer, "One Armed Bandits"

Daisy Duke is a well-meaning though rather naive, often scantily dressed rogue Southern belle with an outgoing and feisty personality. Brought up by her wise Uncle Jesse, Daisy always believes in doing the right thing and helping others. She has a habit of landing herself and her family in trouble, often falling in love with criminals despite the warnings of her family. Although she is not quite as good at driving as her cousins Bo and Luke, Daisy is a relatively talented driver. She often gets involved in the ongoing war between Boss Hogg and her family, helping out where she can. Her job at The Boar's Nest gives her the opportunity to eavesdrop on private conversations between Boss, Sheriff Rosco and various cohorts, often discovering important information that she can pass on to Uncle Jesse and the Duke boys.

Daisy never finds a long-lasting beau over the course of the series, though Deputy Enos Strate has a long-running crush on her that spans the life of the series. This crush is largely unrequited, although Daisy is aware of it and often displays genuine care and concern for the likeable Enos. In the penultimate episode of the show's run, "Enos and Daisy's Wedding," the pair plan to hastily get married as a way to avoid Daisy having to testify against Enos, though the situation is eventually resolved before the wedding takes place. In his recurring appearances during the show (typically once per season), Boss Hogg's nephew Hughie also displays a romantic interest in Daisy (he actually feels no genuine respect or affection for her, however; he merely harbors a selfish lust for her beauty), although Daisy loathes the idea, and there is occasionally a vague hint of a possible previous romantic falling-out between the pair. The fourth season opening episode, "Mrs. Daisy Hogg," sees Daisy falling in love and planning to marry another one of Boss's nephews, Jaimie Lee Hogg (the character's only appearance in the series), although before the wedding she realises he is a villain and the marriage is called off. Similarly, in the second-season episode "Duke of Duke," Daisy becomes attracted to a visiting English Duke claiming to be a distant relation of the Duke clan; she eventually finds out he is a con man and again the romance is over.