The Freedom Rides Museum is located at 210 South Court Street in Montgomery, Alabama, in the building which was until 1995 the Montgomery Greyhound Bus Station. It was the site of a violent attack on participants in the 1961 Freedom Ride during the Civil Rights Movement. The May 1961 assaults, carried out by a mob of white protesters who confronted the civil rights activists, "shocked the nation and led the Kennedy Administration to side with civil rights protesters for the first time."

The property is no longer used as a bus station, but the building was saved from demolition and its façade has been restored. The site was leased by the Alabama Historical Commission and a historical marker was located in front of the building. In 2011, a museum was opened inside the building, and it was listed on the National Register of Historic Places. The museum won a national preservation award from the National Trust for Historic Preservation in 2012.

In 1950, Greyhound Lines retained architect W.S. Arrasmith to build a new bus station in Montgomery, Alabama, to replace an earlier station on North Court Street. Incorporating a streamlined style and vertical "Greyhound" name in neon, it is an unassuming example of Greyhound bus stations in that time, derived from a standard plan and built for $300,000. The station opened in August, 1951.

The bus station is significant only in its relationship to the events of the single day of May 20, 1961. The building had a door labelled "Colored Entrance"; African Americans entered through it directly into the bus bay, accessing interior of the segregated terminal from the rear. The site at 210 Court Street placed the station directly behind Montgomery's U.S. District Courthouse on Lee Street.

Freedom Riders were civil rights activists who rode interstate buses into the segregated southern United States, in 1961 and subsequent years, to challenge the non-enforcement of the United States Supreme Court decisions which had ruled segregated public buses to be unconstitutional.

As organized by the Congress of Racial Equality (CORE) for May 1961, the rides would have mixed pairs of riders sit side by side on Greyhound and Trailways buses crossing the American South from Washington, D.C. to New Orleans, between May 4 and May 17. Alabama stops were planned for Anniston, Birmingham, and Montgomery, during the final leg that ran from Atlanta, Georgia to New Orleans.

In Anniston, a mob of angry whites violently attacked the Greyhound bus and set it on fire; the riders were severely beaten. The Trailways bus arrived an hour later and was boarded in Anniston by Ku Klux Klan members who beat up the Freedom Riders. It was also attacked in Birmingham, and several riders (including James Peck) were beaten in front of the press. Reports of the violence reached U.S. Attorney General Robert F. Kennedy, who urged restraint on the part of Freedom Riders and sent an assistant, John Seigenthaler, to Birmingham. CORE agreed to halt the Freedom Ride in Birmingham on May 14, with the remaining riders flying to New Orleans.

Diane Nash, of the Nashville Student Movement (and a member of the Student Nonviolent Coordinating Committee) and others were undeterred, and 21 young students, including John Lewis, took the place of the original riders for a leg of the Freedom Ride to Montgomery (the ultimate destination was Jackson, Mississippi). All but one (Ruby Doris Smith, from Atlanta) were from Nashville, Tennessee, and many were from Fisk University. Greyhound had initially refused to allow any of their drivers to drive the bus; after an angry intervention by Robert F. Kennedy, and with an escort of state troopers provided by Floyd Mann, the Alabama Director of Public Safety, the bus left Birmingham for Montgomery on May 20.