Hebrew cantillation, trope, trop, or te'amim is the manner of chanting ritual readings from the Hebrew Bible in synagogue services. The chants are written and notated in accordance with the special signs or marks printed in the Masoretic Text of the Bible, to complement the letters and vowel points.

These marks are known in English as 'accents' (diacritics), 'notes' or trope symbols, and in Hebrew as taʿamei ha-mikra (טעמי המקרא) or just teʿamim (טעמים). Some of these signs were also sometimes used in medieval manuscripts of the Mishnah. The musical motifs associated with the signs are known in Hebrew as niggun or neginot (not to be confused with Hasidic nigun) and in Yiddish as trop (טראָפּ): the word trope is sometimes used in Jewish English with the same meaning.

There are multiple traditions of cantillation. Within each tradition, there are multiple tropes, typically for different books of the Bible and often for different occasions. For example, different chants may be used for Torah readings on Rosh Hashana and Yom Kippur than for the same text on a normal Shabbat.

Three systems of Hebrew punctuation (including vowels and cantillation symbols) have been used: the Babylonian, the Jerusalem, and the Tiberian, only the last of which is used today.

Babylonian Biblical manuscripts from the Geonic period contain no cantillation marks in the current sense, but small Hebrew letters are used to mark significant divisions within a verse. Up to eight different letters are found, depending on the importance of the break and where it occurs in the verse: these correspond roughly to the disjunctives of the Tiberian system. For example, in some manuscripts the letter tav, for tevir (break), does duty for both Tiberian tevir and zaqef. In general there are no symbols for the conjunctives, though some late manuscripts use the Tiberian symbols for these. There is also no equivalent for low-grade disjunctives such as telisha gedolah: these are generally replaced by the equivalent of zaqef or revia.

Nothing is known of the musical realization of these marks, but it seems likely that they represent breaks or variations in a set melody applied to each verse. (A somewhat similar system is used in manuscripts of the Qur'an to guide the reader in fitting the chant to the verse: see Qur'an reading.)

This system is reflected in the cantillation practices of the Yemenite Jews, who now use the Tiberian symbols, but tend to have musical motifs only for the disjunctives and render the conjunctives in a monotone. It is notable that the Yemenite Jews have only eight disjunctive motifs, thus clearly reflecting the Babylonian notation. The same is true of the Karaite mode for the haftarah; while in the Sephardi haftarah modes different disjunctives often have the same or closely similar motifs, reducing the total number of effective motifs to something like the same number.

The Babylonian system, as mentioned above, is mainly concerned with showing breaks in the verse. Early manuscripts from Judaea and Galilee, by contrast, are mainly concerned with showing phrases: for example the tifcha-etnachta, zarqa-segolta and pashta-zaqef sequences, with or without intervening unaccented words. These sequences are generally linked by a series of dots, beginning or ending with a dash or a dot in a different place to show which sequence is meant. Unaccented words (which in the Tiberian system carry conjunctives) are generally shown by a dot following the word, as if to link it to the following word. There are separate symbols for more elaborate tropes like pazer and telisha gedolah.