Holyrood distillery is a distillery in Edinburgh, Scotland. It opened in 2019 as the first new distillery in the city in nearly one hundred years. The distillery building was once the Goods shed building of St Leonards railway station.

The company produces Scotch whisky and gin. Whisky production has experimented with heirloom varieties of barley.

The distillery was founded by couple Rob and Kelly Carpenter, and David Robertson. The Carpenters had previously founded a branch of the Scotch Malt Whisky Society in Canada; Robertson had previously worked for Macallan.

The company's bid to take over the lease of the council-owned St. Leonard's Engine Shed was approved by City of Edinburgh Council in 2015, and planning permission for conversion into a distillery was granted the following year. Construction on the site began in 2018, after the company raised £5.8 million in funding from the Scottish National Investment Bank and private investors.

In 2019, the distillery opened to the public for tours.

The distillery ran a week of free-of-charge tours for its fifth anniversary in July 2024. Later that year, the distillery released an exclusive whisky for train company Lumo, launched at Edinburgh Waverley railway station.

Holyrood Distillery released its first single malt whisky, Arrival, in October 2023. The name was a reference to the distillery building's previous role as a train station, and the bottle was designed to resemble a beer bottle, inspired by a local glassworks. The first bottle of Arrival was auctioned by the Worshipful Company of Distillers in aid of a youth charity.

The distillery then released a 'founding series' of three single malts: Embra, a peated whisky; Ambir, made with specialty malts, and Pitch, made with speciality yeast. A limited edition series of expressions, Re-Rack, was launched in 2025, which were themed around different casks.