Horcajo Medianero is a village and municipality in the province of Salamanca, western Spain, part of the autonomous community of Castile-Leon. It is located 48 kilometres (30 mi) from the provincial capital city of Salamanca and has a population of 329 people.
The municipality covers an area of 53 km2 (20 sq mi). It lies 1,008 metres (3,307 ft) above sea level and the postal code is 37860.