Disney's House of Mouse (or simply House of Mouse) is an American crossover animated television series produced by Walt Disney Television Animation that originally aired on ABC and Toon Disney from January 13, 2001, to October 24, 2003, with 52 episodes. The show focuses on Mickey Mouse and his friends running a cartoon theater dinner club in the fictional setting of ToonTown, catering to many characters from Disney cartoons and animated movies while showcasing a variety of their cartoon shorts. The series is named after a common nickname or epithet for the Walt Disney Company.

The animated series is a spin off of the series Mickey Mouse Works, and featured many of the series' shorts as well as selection of brand new shorts, and classic Mickey Mouse, Goofy and Donald Duck shorts from the 1930s–50s.

During its time, the animated series held two nominations for awards, while select cast members won two awards for their performances as characters in House of Mouse. The series featured two direct-to-video films – Mickey's Magical Christmas: Snowed in at the House of Mouse and Mickey's House of Villains – along with an all-night marathon of the House of Mouse, aired on Toon Disney in September 2002 under the title "Night of 1,000 Toons".

The basic premise of the show focuses on Mickey Mouse and his friends operating a dinner theater club in downtown ToonTown. Considered a popular venue by the residents, the club is frequented by a host of characters from Disney animated properties; a feature of the series is that every film produced by Walt Disney Feature Animation prior to the start of the series (between and including Snow White and the Seven Dwarfs and Atlantis: The Lost Empire, with the exception of the CGI Dinosaur) are featured in the series. Such characters mostly appear as paying guests of the club, and would often have speaking roles, although a number sometimes operate as performers for the club. The series also includes many relatively obscure and otherwise rarely used Disney characters, often with speaking parts for the first time – for example, Li'l Bad Wolf and April, May and June, who had appeared very often in Disney comic books but never before in an animated cartoon, made their animated debuts on House of Mouse. The show also featured some cameos by characters created for other television cartoons such as Pepper Ann and theme park attractions like The Haunted Mansion, but these appearances were few and far between.

Each episode focuses on a story involving Mickey and his associates facing an issue during an evening's operation of the club, and their efforts to overcome it – the most common plot for episodes involves the group dealing with a serious problem caused by Pete in his attempts to shut down the club and use it for his own gains. These stories, often involving farcical mishaps, tended to act as a wraparound for the cartoon shorts played in between scenes, the theme of the story contributing towards the story-lines of the cartoon shorts shown in the episode. Cartoon shorts played in episodes focused on elements from classic theatrical cartoons of the 1930s, 1940s and 1950s, though most were reruns from Mickey Mouse Works, and featured a simple story.

Then vice president of Disney Television Animation at the time, Barry Blumberg, wanted to produce a series that worked as a better format for Mickey Mouse Works. Bobs Gannaway and Tony Craig aimed to have the series reintroduce Disney characters to a new generation who were only familiar with them via marketing and "homogenized theme-park figures". They wanted to avoid making Mickey Mouse "hip" or "edgy" and retain the characters' personalities as closely as possible. The series was notable in that it allowed the characters to be played with more loosely as Craig stated, "Everybody thought it was really funny to loosen up a bit and let the characters be who they were and have some fun, instead of being so stiff". The crew was not allowed to use any of the characters from Tarzan due to licensing issues. Gannaway and Craig also deliberately tried to avoid using characters from The Hunchback of Notre Dame due to them being "inherently dramatic", nevertheless, Tantor, Quasimodo and a few other characters from The Hunchback of Notre Dame would make a couple of cameos in the series. There were other limits for this program as well, which forbids the use of Pixar characters due to Disney not owning content from Pixar prior to 2006 and King Louie following a legal dispute. This even omits characters created for the direct-to-video sequels due to the show’s primarily focus on characters that are of Disney canon with a few exceptions.^{[citation needed]}

The show was produced by Walt Disney Television, and originally aired from 2001 to 2003, running for 52 episodes. The show is one of many Disney cartoon series made in the widescreen HD format. Like with Mickey Mouse Works, the animation was outsourced to Toon City in the Philippines. Unlike that series, it was also outsourced to Walt Disney Animation Australia and Walt Disney Animation Japan.

The theme song is performed by Brian Setzer.