Interstate 16 (I-16), also known as Jim Gillis Historic Savannah Parkway, is an east–west Interstate Highway located entirely within the US state of Georgia. It carries the hidden designation of State Route 404 (SR 404) for its entire length. I-16 travels from downtown Macon, at an interchange with I-75 and SR 540 to downtown Savannah at Montgomery Street (exit 167B). It also passes through or near the communities of Dublin, Metter, and Pooler. I-16's unsigned designation of SR 404 has a spur that is signed in Savannah.

The westernmost segment in Macon is part of the Fall Line Freeway, a highway that connects Columbus and Augusta. This segment may also be incorporated into the proposed eastern extension of I-14, which is currently entirely within Central Texas and may be extended to Augusta.

All of I-16 is included as part of the National Highway System, a system of routes determined to be the most important for the nation's economy, mobility, and defense.

I-16 begins at an interchange with I-75/SR 540, just northwest of downtown Macon, in Bibb County. Here, it begins a concurrency with SR 540. The Interstate and SR 540 proceed southeast, traveling just east of the downtown area. They cross over the Ocmulgee River and then have an interchange with US 23/US 129/SR 49 (Spring Street/North Avenue). Then, they have a partial interchange with SR 22 (2nd Street) that is only accessible from the westbound lanes. Almost immediately is an interchange with US 80/SR 87 (Martin Luther King Jr. Boulevard/Coliseum Drive). At this intersection, SR 540 departs the concurrency to the north-northeast. Within the eastern part of this interchange, the highway travels under a railroad bridge that carries railroad tracks of Norfolk Southern Railway. In the east-central part of Macon, I-16 travels through Ocmulgee Mounds National Historical Park but without direct access. Visitors need to first exit at the US 80/SR 87 exit. On the southern edge of the national monument, it crosses over Walnut Creek. It then travels on a bridge over some railroad tracks of CSX and Boggy Branch. After leaving Macon, I-16 curves to the south-southeast and has an interchange with US 23/U.S. Route 129 Alternate (US 129 Alt.)/SR 87 (Ocmulgee East Boulevard). In the interchange, the highway crosses over Swift Creek. It crosses over Stone Creek before entering Twiggs County.

I-16 curves back to the southeast and has an interchange with Sgoda Road. It crosses over Flat Creek and then has an interchange with Jeffersonville and Bullard roads. It curves to the east-southeast and crosses over Savage and Turvin creeks. It then curves back to the southeast. The highway has an interchange with SR 96. Almost immediately, it crosses over Richland Creek. It then has an interchange with SR 358. I-16 curves to the east-southeast and enters Bleckley County just before it has an interchange with SR 112 just south of Allentown. It then crosses over Rocky Creek just before entering Laurens County. The Interstate curves back to the southeast and crosses under SR 278 before it travels south of Montrose. It crosses over Bay Branch just before an interchange with SR 26. It then enters the southwestern part of Dudley. There, it has an interchange with SR 338. I-16 curves back to the east-southeast and crosses over Little Rocky Creek just before a rest area. Just to the west-northwest of a crossing of Turkey Creek, the westbound lanes have a rest area. These two rest areas are the only ones along the entire length of I-16. On the southwestern edge of Dublin, the highway has an interchange with SR 257. On the southern edge of the city are interchanges with US 319/US 441/SR 31 and SR 19 (Martin Luther King Jr. Drive). It then crosses over the Oconee River. It has an interchange with SR 199 (Old River Road) just before a crossing of Pughes Creek. Southeast of that is a crossing of Red Hill Creek. Just south of Rockledge, the highway crosses over Mercer Creek. On the eastern edge of the creek, it enters Treutlen County.

I-16 curves to the east-northeast and crosses over some railroad tracks of CSX before an interchange with SR 29. It immediately curves back to the east-southeast. It crosses over Red Bluff Creek. Then is an interchange with SR 15/SR 78. The highway travels south of Sand Hill Lake before curving to the east-northeast. It crosses over Pendleton Creek and travels under a bridge that carries SR 86. Then, it begins to curve to the southeast. It has an interchange with US 221/SR 56. It crosses over Reedy Creek and curves to the east-southeast. It has an interchange with SR 297. At the overpass for SR 297, the highway enters Emanuel County.

After the SR 297 interchange, I-16 heads more to the southeast. It curves to a nearly due east direction and crosses over the Ohoopee River. Just after crossing over some railroad tracks of Norfolk Southern Railway, it enters the city limits of Oak Park. It curves to the southeast and has an interchange with US 1/SR 4/SR 46. After this interchange, the highway begins to parallel SR 46. It curves to the east-southeast and crosses over Jacks Creek. Then, it enters Candler County.

I-16 has an interchange with SR 57 (Aline Road). It crosses over Wolfe Creek and then heads to the east-northeast. It crosses over the Canoochee River and curves to a nearly due east direction. It crosses over Sams Creek before entering Metter. As soon as it enters Metter, it passes the Metter Municipal Airport. Right after the airport is an interchange with SR 23/SR 121. On the southeastern edge of Metter, I-16 travels under a bridge that carries SR 129. It crosses over 15 Mile Creek and then curves to the southeast. It crosses over Tenmile Creek and has an interchange with Pulaski–Excelsior Road just before entering Bulloch County.