The Kentucky General Assembly, also called the Kentucky Legislature, is the state legislature of the U.S. state of Kentucky. It comprises the Kentucky Senate and the Kentucky House of Representatives.

The General Assembly meets annually in the state capitol building in Frankfort, convening on the first Tuesday after the first Monday in January. In even-numbered years, sessions may not last more than 60 legislative days, and cannot extend beyond April 15. In odd-numbered years, sessions may not last more than 30 legislative days, and cannot extend beyond March 30. Special sessions may be called by the Governor of Kentucky at any time and for any duration.

The first meeting of the General Assembly occurred in 1792, shortly after Kentucky was granted statehood. Legislators convened in Lexington, the state's temporary capital. Among the first orders of business was choosing a permanent state capital. In the end, the small town of Frankfort, with their offer to provide a temporary structure to house the legislature and a cache of materials for constructing a permanent edifice, was chosen, and the state's capital has remained there ever since.

After women gained suffrage in Kentucky, Mary Elliott Flanery was elected to the Kentucky House of Representative from the 89th District, representing Boyd County, Kentucky. When Flanery took her seat in January 1922, she was the first female state legislator elected in Kentucky and the first female legislator elected south of the Mason–Dixon line.

Operation Boptrot led to the conviction of more than a dozen legislators between 1992 and 1995. The investigation also led to reform legislation being passed in 1993.

Due to the strong Southern Unionist sympathies of a large portion of the Commonwealth's citizens and elected officials, Kentucky remained officially neutral during the Civil War. Even so, a group of Confederate sympathizers met in Russellville representing 68 Kentucky counties in the western and central parts of the state in November 1861, to establish a Confederate government for the state. The group established a Confederate state capital in Bowling Green controlling half the state early in the war, but never successfully displaced the elected General Assembly in Frankfort.

The General Assembly played a decisive role in the disputed gubernatorial election of 1899. Initial vote tallies had Republican William S. Taylor leading Democrat William Goebel by a scant 2,383 votes. The General Assembly, however, wielded the final authority in election disputes. With a majority in both houses, the Democrats attempted to invalidate enough votes to give the election to Goebel. During the contentious days that followed, an unidentified assassin shot Goebel as he approached the state capitol.

As Goebel hovered on the brink of death, chaos ensued in Frankfort, and further violence threatened. Taylor, serving as governor pending a final decision on the election, called out the militia and ordered the General Assembly into a special session, not in Frankfort, but in London, Kentucky, a Republican area of the state. The Republican minority naturally heeded the call and headed to London. Democrats predictably resisted the call, many retiring to Louisville instead. Both factions claimed authority, but the Republicans were too few in number to muster a quorum.