Last Gasp is a San Francisco–based book publisher with a lowbrow art and counterculture focus. Owned and operated by Ron Turner, for most of its existence Last Gasp was a publisher, distributor, and wholesaler of underground comix and books of all types.

Last Gasp was established in 1970. Although the company came onto the scene a bit later than some of the other underground publishers, Last Gasp continued publishing comics far longer than most of its competitors. In addition to publishing notable original titles like Slow Death, Wimmen's Comix, Binky Brown Meets the Holy Virgin Mary, and Weirdo, it also picked up the publishing reins of important titles—such as Zap Comix and Young Lust—from rivals who had gone out of business.

Although Last Gasp no longer publishes "floppy" comics; the company continues to publish art and photography books, graphic novels, fiction, and poetry, producing 10–15 new titles per year.

Last Gasp Eco Funnies was founded in Berkeley, California, in 1970 by San Francisco State University graduate student Ronald E. Turner, with the help of Gary Arlington, to publish the ecologically-themed comics title Slow Death Funnies (in conjunction with the first Earth Day). Last Gasp followed Slow Death Funnies with the all-female anthology It Ain't Me, Babe, spearheaded by Trina Robbins.

Last Gasp was incorporated on September 11, 1971. In the time period 1971–1973, Last Gasp published Air Pirates Funnies #1–2 and a number of other Air Pirates-related titles, including The Tortoise and the Hare, Dopin' Dan #1–3, and Merton of the Movement. (Turner insists that he only served as an advisor to the Air Pirates collective, despite them crediting his company as "publisher.") Considered to be an "enabler" of the Air Pirates infamous Walt Disney parodies, Turner's name was added to Disney's lawsuit against the collective. Turner quickly settled with Disney, but Air Pirates Dan O'Neill, Bobby London, and Ted Richards continued fighting, in a case that dragged on for years.

The company's success with Slow Death and It Ain't Me, Babe enabled Last Gasp to expand into distribution, in addition to publishing. The company soon became a major part of the underground comix movement. Sociopolitical themes were explored in Last Gasp series such as Guy Colwell's Inner City Romance (1972–1979), which portrayed gritty urban tales; Armageddon (1973), which focused on anarcho-capitalism; and Anarchy Comics (1978), which focused on left-wing politics. In addition to publishing Wimmen's Comix for much of its run, Last Gasp published a number of other comix with feminist themes, including It Ain't Me, Babe, Tits & Clits Comix, Twisted Sisters #1, and Good Girls.

In 1972, Last Gasp published Justin Green's seminal autobiographical comic Binky Brown Meets the Holy Virgin Mary.

Beginning in 1972, Last Gasp began publishing ongoing titles moving over from other companies; beginning with Young Lust, and then Rand Holmes' Harold Hedd. In 1977, the company picked up Joyce Farmer and Lyn Chevely's Tits & Clits Comix, publishing that series until 1987. Last Gasp published the final three issues of San Francisco Comic Book in 1980–1983, and the latter half of Zap Comix' run from 1982 to 2005.