The following is a collection of enemies and supervillains appearing in American comic books published by DC Comics. These characters are depicted as adversaries of the superhero Aquaman and his family and allies.

Since Aquaman first appeared in More Fun Comics #73 (November 1941), as with most superheroes, a cast of recurring enemies to Aquaman and his family have been introduced throughout the years. Some of the character's most notable villains include his archenemy, the mercenary Black Manta; his own half-brother the Ocean Master; the humanoid sharks King Shark and Orca; the pirate the Scavenger; Mera's twin sister Siren; and the Trench species, among others.

The following fictional characters are listed in alphabetical order by the name of their supervillain persona. Each character's first appearance and brief biographies of each fictional character are also listed, staying to their fictional histories and characteristics in the DC Universe. Sometimes more than one fictional character will share a supervillain persona. In those cases, those will be divided into multiple sections.

A number of villains from the comic books have appeared in Aquaman-related media.