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This is a list containing the Billboard Hot Latin Tracks number-ones of 2000.
| Issue date | Song | Artist(s) | Ref. |
|---|---|---|---|
| January 1 | "Ritmo Total" | Enrique Iglesias | [1] |
| January 8 | "Fruta Fresca" | Carlos Vives | [2] |
| January 15 | "Te Quiero Mucho" | Los Rieleros del Norte | [3] |
| January 22 | "Fruta Fresca" | Carlos Vives | [4] |
| January 29 | [5] | ||
| February 5 | "Desnuda" | Ricardo Arjona | [6] |
| February 12 | "Fruta Fresca" | Carlos Vives | [7] |
| February 19 | "Desnuda" | Ricardo Arjona | [8] |
| February 26 | "Fruta Fresca" | Carlos Vives | [9] |
| March 4 | [10] | ||
| March 11 | "Que Alguien Me Diga" | Gilberto Santa Rosa | [11] |
| March 18 | [12] | ||
| March 25 | [13] | ||
| April 1 | "A Puro Dolor" | Son by Four | [14] |
| April 8 | [15] | ||
| April 15 | [16] | ||
| April 22 | [17] | ||
| April 29 | [18] | ||
| May 6 | [19] | ||
| May 13 | [20] | ||
| May 20 | [21] | ||
| May 27 | [22] | ||
| June 3 | [23] | ||
| June 10 | "No Me Dejes de Querer" | Gloria Estefan | [24] |
| June 17 | "Entre El Mar Y Una Estrella" | Thalía | [25] |
| June 24 | "A Puro Dolor" | Son by Four | [26] |
| July 1 | "Muy Dentro de Mí" | Marc Anthony | [27] |
| July 8 | [28] | ||
| July 15 | [29] | ||
| July 22 | "A Puro Dolor" | Son by Four | [30] |
| July 29 | [31] | ||
| August 5 | [32] | ||
| August 12 | "Júrame" | Giselle | [33] |
| August 19 | "A Puro Dolor" | Son by Four | [34] |
| August 26 | [35] | ||
| September 2 | [36] | ||
| September 9 | "Imaginame Sin Ti" | Luis Fonsi | [37] |
| September 16 | [38] | ||
| September 23 | "Como Me Duele Perderte" | Gloria Estefan | [39] |
| September 30 | [40] | ||
| October 7 | "Cuando" | Ricardo Arjona | [41] |
| October 14 | "Ven Conmigo (Solamente Tú)" | Christina Aguilera | [42] |
| October 21 | [43] | ||
| October 28 | "Ven a Mí" | Oscar De La Hoya | [44] |
| November 4 | "She Bangs" | Ricky Martin | [45] |
| November 11 | "A Puro Dolor" | Son by Four | [46] |
| November 18 | [47] | ||
| November 25 | [48] | ||
| December 2 | "Yo Te Amo" | Chayanne | [49] |
| December 9 | [50] | ||
| December 16 | [51] | ||
| December 23 | [52] | ||
| December 30 | "Cuando Seas Mía (Miss Me So Bad)" | Son By Four | [53] |
